Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Mouse monoclonal [5A3] to NTF2
- Suitable for: WB, ICC
- Reacts with: Mouse, Rat, Human
Product nameAnti-NTF2 antibody [5A3]
See all NTF2 primary antibodies
DescriptionMouse monoclonal [5A3] to NTF2
Tested applicationsSuitable for: WB, ICCmore details
Unsuitable for: IHC-P
Species reactivityReacts with: Mouse, Rat, Human
Recombinant fragment. This information is considered to be commercially sensitive.
- WB: HeLa, HEK-293, HepG2, K562, IMR-32, F9 and L6 whole cell lysate. ICC: HeLa cells.
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact firstname.lastname@example.org.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab254146 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Predicted molecular weight: 14 kDa.|
FunctionFacilitates protein transport into the nucleus. Interacts with the nucleoporin p62 and with Ran. Acts at a relatively late stage of nuclear protein import, subsequent to the initial docking of nuclear import ligand at the nuclear envelope. Could be part of a multicomponent system of cytosolic factors that assemble at the pore complex during nuclear import.
Sequence similaritiesContains 1 NTF2 domain.
- Information by UniProt
- NTF 2 antibody
- NTF-2 antibody
- NTF2 antibody
All lanes : Anti-NTF2 antibody [5A3] (ab254146) at 1/1000 dilution
Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate
Lane 2 : HEK-293 (human embryonic kidney epithelial cell), whole cell lysate
Lane 3 : HepG2 (human hepatocellular carcinoma epithelial cell), whole cell lysate
Lane 4 : K562 (human chronic myelogenous leukemia lymphoblast), whole cell lysate
Lane 5 : IMR-32 (human neuroblastoma neuroblast), whole cell lysate
Lane 6 : F9 (mouse embryonal carcinoma epithelial cell), whole cell lysate
Lane 7 : L6 (rat skeletal muscle myoblast), whole cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution
Predicted band size: 14 kDa
Exposure time: 8 seconds
Blocking/Dilution buffer: 5% NFDM/TBST.
Immunocytochemical analysis of 4% paraformaldehyde-fixed, 100% methanol-permeabilized HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling NTF2 with ab254146 at 1/50 dilution, followed by ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing nuclear and cytoplasmic staining in HeLa cells. ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/200 dilution, followed by ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) at a 1/1000 dilution (Red). The nuclear counterstain was DAPI (Blue).
Negative control 1: ab254146 at a 1/50 dilution followed by ab150080 at a 1/200 dilution.
Negative control 2: ab179513 at a 1/200 dilution followed by ab150113 at a 1/1000 dilution.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab254146 has been referenced in 1 publication.
- Steggerda SM et al. Monoclonal antibodies to NTF2 inhibit nuclear protein import by preventing nuclear translocation of the GTPase Ran. Mol Biol Cell 11:703-19 (2000). PubMed: 10679025