Overview

  • Product name

    Anti-NTH1 antibody [2660C1a]
    See all NTH1 primary antibodies
  • Description

    Mouse monoclonal [2660C1a] to NTH1
  • Host species

    Mouse
  • Tested applications

    Suitable for: ICC/IF, WB, Dot blotmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant N-terminal fragment of human NTH1

  • Positive control

    • Recombinant human NTH1. Whole cell lysates from human HEK293, mouse NIH3T3 and rat F2408 cells.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.05% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • Purity

    Protein G purified
  • Purification notes

    ab70726 was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum and filtered through a 0.22µm membrane.
  • Clonality

    Monoclonal
  • Clone number

    2660C1a
  • Isotype

    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab70726 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
WB Use a concentration of 0.2 - 2 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 34 kDa).
Dot blot Use at an assay dependent concentration.

Target

  • Function

    Has both an apurinic and/or apyrimidinic endonuclease activity and a DNA N-glycosylase activity. Incises damaged DNA at cytosines, thymines and guanines. Acts on a damaged strand, 5' from the damaged site. Required for the repair of both oxidative DNA damage and spontaneous mutagenic lesions.
  • Tissue specificity

    Widely expressed with highest levels in heart and lowest levels in lung and liver.
  • Sequence similarities

    Belongs to the Nth/MutY family.
  • Developmental stage

    Expression levels are regulated during the cell cycle with increased levels during early and mid S-phase.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • Bifunctional DNA N glycoslyase/DNA (apurinic or apyrimidinic site) lyase antibody
    • DNA glycoslyase/AP lyase antibody
    • Endonuclease III like protein 1 antibody
    • Endonuclease III-like protein 1 antibody
    • hNTH1 antibody
    • NTH 1 antibody
    • nth endonuclease III like 1 (E. coli) antibody
    • NTH endonuclease III Like 1 antibody
    • NTH1 antibody
    • NTHL 1 antibody
    • Nthl1 antibody
    • NTHL1_HUMAN antibody
    • OCTS 3 antibody
    • OCTS3 antibody
    see all

Images

  • All lanes : Anti-NTH1 antibody [2660C1a] (ab70726) at 0.2 µg/ml

    Lane 1 : Whole cell lysate from human HEK293 cells
    Lane 2 : Whole cell lysate from mouse NIH3T3 cells
    Lane 3 : Whole cell lysate from rat F2408 cells

    Lysates/proteins at 25 µg per lane.

    Predicted band size: 34 kDa
    Observed band size: 37 kDa
    why is the actual band size different from the predicted?

  • Anti-NTH1 antibody [2660C1a] (ab70726) at 0.2 µg/ml + immunised recombinant human NTH1 protein

    Predicted band size: 34 kDa
    Observed band size: 35 kDa why is the actual band size different from the predicted?

  • ab70726 staining NTH1 in HeLa cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.2% Triton X-100 and blocked with 2% BSA for 45 minutes at room temperature. Samples were incubated with primary antibody (1/300 in PBS + 2% BSA) for 14 hours at 4°C. An Alexa Fluor® 594-conjugated goat anti-mouse IgG polyclonal (1/500) was used as the secondary antibody.

    See Abreview

References

This product has been referenced in:

  • Williams SC & Parsons JL NTH1 Is a New Target for Ubiquitylation-Dependent Regulation by TRIM26 Required for the Cellular Response to Oxidative Stress. Mol Cell Biol 38:N/A (2018). Read more (PubMed: 29610152) »
  • Limpose KL  et al. Overexpression of the base excision repair NTHL1 glycosylase causes genomic instability and early cellular hallmarks of cancer. Nucleic Acids Res 46:4515-4532 (2018). Read more (PubMed: 29522130) »
See all 2 Publications for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 45 minute(s) · Concentration: 2% · Temperature: RT°C
Sample
Human Cell (HeLa cells)
Specification
HeLa cells
Permeabilization
Yes - 0.2% Triton-X100
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted May 15 2014

Application
Western blot
Sample
Mouse Cell lysate - whole cell (Mouse embryonic fibroblasts)
Loading amount
40 µg
Specification
Mouse embryonic fibroblasts
Gel Running Conditions
Reduced Denaturing (4-20% Tris-Gly gel)
Blocking step
LI-COR® Odyssey® Blocking Buffer as blocking agent for 45 minute(s) · Concentration: 50% · Temperature: RT°C

Abcam user community

Verified customer

Submitted Apr 09 2012

Application
Western blot
Sample
Human Cell lysate - whole cell (HeLa cells)
Loading amount
20 µg
Specification
HeLa cells
Gel Running Conditions
Reduced Denaturing (4-16% Tris-Gly gel)
Blocking step
LI-COR® Odyssey® Blocking Buffer as blocking agent for 45 minute(s) · Concentration: 50% · Temperature: RT°C

Abcam user community

Verified customer

Submitted Apr 09 2012

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