Overview

  • Product name
  • Description
    Rabbit polyclonal to NTH1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human NTH1 (internal sequence). The exact sequence is proprietary.
    Database link: P78549

  • Positive control
    • ICC/IF: A549 cells.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab228489 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100 - 1/1000.

Target

  • Function
    Has both an apurinic and/or apyrimidinic endonuclease activity and a DNA N-glycosylase activity. Incises damaged DNA at cytosines, thymines and guanines. Acts on a damaged strand, 5' from the damaged site. Required for the repair of both oxidative DNA damage and spontaneous mutagenic lesions.
  • Tissue specificity
    Widely expressed with highest levels in heart and lowest levels in lung and liver.
  • Sequence similarities
    Belongs to the Nth/MutY family.
  • Developmental stage
    Expression levels are regulated during the cell cycle with increased levels during early and mid S-phase.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Bifunctional DNA N glycoslyase/DNA (apurinic or apyrimidinic site) lyase antibody
    • DNA glycoslyase/AP lyase antibody
    • Endonuclease III like protein 1 antibody
    • Endonuclease III-like protein 1 antibody
    • hNTH1 antibody
    • NTH 1 antibody
    • nth endonuclease III like 1 (E. coli) antibody
    • NTH endonuclease III Like 1 antibody
    • NTH1 antibody
    • NTHL 1 antibody
    • Nthl1 antibody
    • NTHL1_HUMAN antibody
    • OCTS 3 antibody
    • OCTS3 antibody
    see all

Images

  • A549 (human lung carcinoma cell line) cells stained for NTH1 (green) using ab228489 at 1:1000 dilution in ICC/IF. Cells were fixed in 4% paraformaldehyde at RT for 15 minutes.

    Blue: Hoechst 33342 staining.

References

ab228489 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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