The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/100 - 1/1000.
Has both an apurinic and/or apyrimidinic endonuclease activity and a DNA N-glycosylase activity. Incises damaged DNA at cytosines, thymines and guanines. Acts on a damaged strand, 5' from the damaged site. Required for the repair of both oxidative DNA damage and spontaneous mutagenic lesions.
Widely expressed with highest levels in heart and lowest levels in lung and liver.
Belongs to the Nth/MutY family.
Expression levels are regulated during the cell cycle with increased levels during early and mid S-phase.