Anti-NTH1 antibody (ab236912)
Key features and details
- Rabbit polyclonal to NTH1
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-NTH1 antibody
See all NTH1 primary antibodies -
Description
Rabbit polyclonal to NTH1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant full length protein corresponding to Human NTH1 aa 1-350. Full length mature protein without the transit peptide.
Database link: P78549 -
Positive control
- WB: NIH/3T3 cell lysate. IHC-P: Human glioma tissue. ICC/IF: HeLa cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
Purity >95% -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab236912 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/5000.
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IHC-P |
1/20 - 1/200.
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ICC/IF |
1/50 - 1/200.
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Notes |
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WB
1/500 - 1/5000. |
IHC-P
1/20 - 1/200. |
ICC/IF
1/50 - 1/200. |
Target
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Function
Has both an apurinic and/or apyrimidinic endonuclease activity and a DNA N-glycosylase activity. Incises damaged DNA at cytosines, thymines and guanines. Acts on a damaged strand, 5' from the damaged site. Required for the repair of both oxidative DNA damage and spontaneous mutagenic lesions. -
Tissue specificity
Widely expressed with highest levels in heart and lowest levels in lung and liver. -
Sequence similarities
Belongs to the Nth/MutY family. -
Developmental stage
Expression levels are regulated during the cell cycle with increased levels during early and mid S-phase. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 4913 Human
- Entrez Gene: 18207 Mouse
- Omim: 602656 Human
- SwissProt: P78549 Human
- SwissProt: O35980 Mouse
- Unigene: 66196 Human
- Unigene: 148315 Mouse
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Alternative names
- Bifunctional DNA N glycoslyase/DNA (apurinic or apyrimidinic site) lyase antibody
- DNA glycoslyase/AP lyase antibody
- Endonuclease III like protein 1 antibody
see all
Images
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Anti-NTH1 antibody (ab236912) at 1/500 dilution + NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution -
Paraffin-embedded human glioma tissue stained for NTH1 using ab236912 at 1/100 dilution in immunohistochemical analysis.
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HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for NTH1 using ab236912 at 1/100 dilution in ICC/IF, followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG (H+L).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab236912 has been referenced in 1 publication.
- Tesfay L et al. Complementary anti-cancer pathways triggered by inhibition of sideroflexin 4 in ovarian cancer. Sci Rep 12:19936 (2022). PubMed: 36402786