RecombinantRabMAb

Recombinant Anti-NTH1 antibody [EPR15930] (ab191413)

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Western blot - Anti-NTH1 antibody [EPR15930] (ab191413)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NTH1 antibody [EPR15930] (ab191413)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NTH1 antibody [EPR15930] (ab191413)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NTH1 antibody [EPR15930] (ab191413)
  • Immunocytochemistry/ Immunofluorescence - Anti-NTH1 antibody [EPR15930] (ab191413)
  • Anti-NTH1 antibody [EPR15930] (ab191413)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR15930] to NTH1
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-NTH1 antibody [EPR15930]
    See all NTH1 primary antibodies

  • Description

    Rabbit monoclonal [EPR15930] to NTH1

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment within Human NTH1 aa 1-150. The exact sequence is proprietary.
    Database link: P78549

  • Positive control

    • Human fetal heart tissue lysate; HeLa, 293 and K562 cell lysates; Human colon, transitional cell carcinoma of bladder and mouse brain tissues; HeLa cells

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR15930

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab191413 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 34 kDa (predicted molecular weight: 34 kDa).
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF 1/150.

Target

  • Function

    Has both an apurinic and/or apyrimidinic endonuclease activity and a DNA N-glycosylase activity. Incises damaged DNA at cytosines, thymines and guanines. Acts on a damaged strand, 5' from the damaged site. Required for the repair of both oxidative DNA damage and spontaneous mutagenic lesions.

  • Tissue specificity

    Widely expressed with highest levels in heart and lowest levels in lung and liver.

  • Sequence similarities

    Belongs to the Nth/MutY family.

  • Developmental stage

    Expression levels are regulated during the cell cycle with increased levels during early and mid S-phase.

  • Cellular localization

    Nucleus.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • Bifunctional DNA N glycoslyase/DNA (apurinic or apyrimidinic site) lyase antibody
    • DNA glycoslyase/AP lyase antibody
    • Endonuclease III like protein 1 antibody
    • Endonuclease III-like protein 1 antibody
    • hNTH1 antibody
    • NTH 1 antibody
    • nth endonuclease III like 1 (E. coli) antibody
    • NTH endonuclease III Like 1 antibody
    • NTH1 antibody
    • NTHL 1 antibody
    • Nthl1 antibody
    • NTHL1_HUMAN antibody
    • OCTS 3 antibody
    • OCTS3 antibody
    see all

Images

  • Western blot - Anti-NTH1 antibody [EPR15930] (ab191413)
    Western blot - Anti-NTH1 antibody [EPR15930] (ab191413)
    All lanes : Anti-NTH1 antibody [EPR15930] (ab191413) at 1/10000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : 293 cell lysate
    Lane 3 : K562 cell lysate
    Lane 4 : Human fetal heart lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 34 kDa
    Observed band size: 34 kDa



    Blocking/ Dilution buffer: 5% NFDM /TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NTH1 antibody [EPR15930] (ab191413)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NTH1 antibody [EPR15930] (ab191413)

    Immunohistochemical analysis of paraffin-embedded human colon tissue labeling NTH1 with ab191413 at 1/100 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with Hematoxylin.

    Negative control utilised PBS instead of ab191413.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NTH1 antibody [EPR15930] (ab191413)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NTH1 antibody [EPR15930] (ab191413)

    Immunohistochemical analysis of paraffin-embedded Human transitional cell carcinoma of bladder tissue labeling NTH1 with ab191413 at 1/100 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with Hematoxylin.

    Negative control utilised PBS instead of ab191413.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NTH1 antibody [EPR15930] (ab191413)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NTH1 antibody [EPR15930] (ab191413)

    Immunohistochemical analysis of paraffin-embedded Mouse brain tissue labeling NTH1 with ab191413 at 1/100 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with Hematoxylin.

    Negative control utilised PBS instead of ab191413.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-NTH1 antibody [EPR15930] (ab191413)
    Immunocytochemistry/ Immunofluorescence - Anti-NTH1 antibody [EPR15930] (ab191413)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% tritonX-100 permeabilized HeLa cells labeling NTH1 with ab191413 at 1/150 dilution, followed by Goat anti rabbit IgG (Alexa Fluor488) secondary antibody at 1/200 dilution (ab150077). Nuclear counter stain is DAPI (blue).

    The two negative controls are ab191413 at 1/250 dilution followed by Goat anti mouse IgG (Alexa Fluor®594) secondary antibody at 1/400 dilution.

  • Anti-NTH1 antibody [EPR15930] (ab191413)
    Anti-NTH1 antibody [EPR15930] (ab191413)

References (2)

Publishing research using ab191413? Please let us know so that we can cite the reference in this datasheet.

ab191413 has been referenced in 2 publications.

  • Li M  et al. APE1 deficiency promotes cellular senescence and premature aging features. Nucleic Acids Res 46:5664-5677 (2018). PubMed: 29750271
  • Maher RL  et al. Human cells contain a factor that facilitates the DNA glycosylase-mediated excision of oxidized bases from occluded sites in nucleosomes. DNA Repair (Amst) 57:91-97 (2017). PubMed: 28709015

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