Overview

  • Product name

    Anti-NTH1 antibody [EPR15930] - BSA and Azide free
    See all NTH1 primary antibodies
  • Description

    Rabbit monoclonal [EPR15930] to NTH1 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, IHC-P, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment within Human NTH1 aa 1-150. The exact sequence is proprietary.
    Database link: P78549

  • Positive control

    • IHC-P: Human colon tissue.
  • General notes

    Ab236143 is the carrier-free version of ab191413. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab236143 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Constituent: PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR15930
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab236143 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB Use at an assay dependent concentration. Detects a band of approximately 34 kDa (predicted molecular weight: 34 kDa).

Target

  • Function

    Has both an apurinic and/or apyrimidinic endonuclease activity and a DNA N-glycosylase activity. Incises damaged DNA at cytosines, thymines and guanines. Acts on a damaged strand, 5' from the damaged site. Required for the repair of both oxidative DNA damage and spontaneous mutagenic lesions.
  • Tissue specificity

    Widely expressed with highest levels in heart and lowest levels in lung and liver.
  • Sequence similarities

    Belongs to the Nth/MutY family.
  • Developmental stage

    Expression levels are regulated during the cell cycle with increased levels during early and mid S-phase.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • Bifunctional DNA N glycoslyase/DNA (apurinic or apyrimidinic site) lyase antibody
    • DNA glycoslyase/AP lyase antibody
    • Endonuclease III like protein 1 antibody
    • Endonuclease III-like protein 1 antibody
    • hNTH1 antibody
    • NTH 1 antibody
    • nth endonuclease III like 1 (E. coli) antibody
    • NTH endonuclease III Like 1 antibody
    • NTH1 antibody
    • NTHL 1 antibody
    • Nthl1 antibody
    • NTHL1_HUMAN antibody
    • OCTS 3 antibody
    • OCTS3 antibody
    see all

Images

  • Immunohistochemical analysis of paraffin-embedded Mouse brain tissue labeling NTH1 with ab191413 at 1/100 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with Hematoxylin.

    Negative control utilised PBS instead of ab191413.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab191413).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemical analysis of paraffin-embedded Human transitional cell carcinoma of bladder tissue labeling NTH1 with ab191413 at 1/100 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with Hematoxylin.

    Negative control utilised PBS instead of ab191413.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab191413).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% tritonX-100 permeabilized HeLa cells labeling NTH1 with ab191413 at 1/150 dilution, followed by Goat anti rabbit IgG (Alexa Fluor488) secondary antibody at 1/200 dilution (ab150077). Nuclear counter stain is DAPI (blue).

    The two negative controls are ab191413 at 1/250 dilution followed by Goat anti mouse IgG (Alexa Fluor®594) secondary antibody at 1/400 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab191413).

  • Immunohistochemical analysis of paraffin-embedded human colon tissue labeling NTH1 with ab191413 at 1/100 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with Hematoxylin.

    Negative control utilized PBS instead of ab191413.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab191413).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

References

ab236143 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab236143.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up