Overview

  • Product name

  • Description

    Rabbit polyclonal to NUB1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide corresponding to Human NUB1 (N terminal) conjugated to keyhole limpet haemocyanin.

  • Positive control

    • Mouse liver lysate. Human hepatocarcinoma tissue.
  • General notes

     This product was previously labelled as NYREN18, Nubbin

     

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Protein G purified
  • Purification notes

    This antibody is purified through a protein G column, eluted out with both high and low pH buffers, neutralized immediately after elution then followed by dialysis against PBS.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab38438 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Detects a band of approximately 71 kDa (predicted molecular weight: 71 kDa).
ELISA 1/1000.
IHC-P 1/50 - 1/100.

Target

  • Function

    Specific down-regulator of the NEDD8 conjugation system. Recruits NEDD8, UBD, and their conjugates to the proteasome for degradation. Isoform 1 promotes the degradation of NEDD8 more efficiently than isoform 2.
  • Tissue specificity

    Widely expressed with lowest expression in the pancreas for isoform 1 and in leukocytes, liver, prostate and skeletal muscle for isoform 2.
  • Sequence similarities

    Contains 3 UBA domains.
  • Cellular localization

    Nucleus. Predominantly nuclear.
  • Information by UniProt
  • Database links

  • Alternative names

    • BS4 antibody
    • NEDD8 ultimate buster 1 antibody
    • Negative regulator of ubiquitin like proteins 1 antibody
    • Negative regulator of ubiquitin-like proteins 1 antibody
    • NUB1 antibody
    • NUB1_HUMAN antibody
    • NUB1L antibody
    • NY REN 18 antibody
    • NYREN18 antibody
    • Renal carcinoma antigen NY-REN-18 antibody
    see all

Images

  • Anti-NUB1 antibody (ab38438) at 1/100 dilution + Mouse liver lysate

    Predicted band size: 71 kDa
    Observed band size: 71 kDa
    Additional bands at: 14 kDa, 23 kDa, 30 kDa, 45 kDa. We are unsure as to the identity of these extra bands.

  • Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with ab38438, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining

References

ab38438 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab38438.
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