Recombinant
RabMAb

Recombinant Anti-NUB1 antibody [EPR10716] (ab171077)

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Overview

  • Product name

    Anti-NUB1 antibody [EPR10716]
    See all NUB1 primary antibodies

  • Description

    Rabbit monoclonal [EPR10716] to NUB1

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, ICC/IF, Flow Cytmore details
    Unsuitable for: IHC-P or IP

  • Species reactivity

    Reacts with: Human
    Does not react with: Mouse, Rat

  • Immunogen

    Synthetic peptide within Human NUB1 aa 1-100 (Cysteine residue). The exact sequence is proprietary.
    Database link: Q9Y5A7

  • Positive control

    • Raji, SHSY5Y, 293T and HeLa cell line lysates. HeLa cells. Permeabilized Raji cells.

  • General notes

     This product was previously labelled as NYREN18, Nubbin

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR10716

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab171077 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 71 kDa.
ICC/IF 1/100 - 1/250.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 
  • Application notes
    Is unsuitable for IHC-P or IP.

    • Target

    • Function

      Specific down-regulator of the NEDD8 conjugation system. Recruits NEDD8, UBD, and their conjugates to the proteasome for degradation. Isoform 1 promotes the degradation of NEDD8 more efficiently than isoform 2.

    • Tissue specificity

      Widely expressed with lowest expression in the pancreas for isoform 1 and in leukocytes, liver, prostate and skeletal muscle for isoform 2.

    • Sequence similarities

      Contains 3 UBA domains.

    • Cellular localization

      Nucleus. Predominantly nuclear.
    • Information by UniProt

    • Database links

      • Alternative names

        • BS4 antibody
        • NEDD8 ultimate buster 1 antibody
        • Negative regulator of ubiquitin like proteins 1 antibody
        • Negative regulator of ubiquitin-like proteins 1 antibody
        • NUB1 antibody
        • NUB1_HUMAN antibody
        • NUB1L antibody
        • NY REN 18 antibody
        • NYREN18 antibody
        • Renal carcinoma antigen NY-REN-18 antibody
        see all

      Images

      • Western blot - Anti-NUB1 antibody [EPR10716] (ab171077)
        Western blot - Anti-NUB1 antibody [EPR10716] (ab171077)
        All lanes : Anti-NUB1 antibody [EPR10716] (ab171077) at 1/1000 dilution

        Lane 1 : Raji cell lysate
        Lane 2 : SHSY5Y cell lysate
        Lane 3 : 293T cell lysate
        Lane 4 : HeLa cell lysate

        Lysates/proteins at 10 µg per lane.

        Predicted band size: 71 kDa

      • Immunocytochemistry/ Immunofluorescence - Anti-NUB1 antibody [EPR10716] (ab171077)
        Immunocytochemistry/ Immunofluorescence - Anti-NUB1 antibody [EPR10716] (ab171077)

        Immunofluorescent staining of HeLa cells labeling NUB1 using ab171077 at 1/100 dilution.

      • Flow Cytometry - Anti-NUB1 antibody [EPR10716] (ab171077)
        Flow Cytometry - Anti-NUB1 antibody [EPR10716] (ab171077)

        Flow cytometric analysis of permeabilized Raji cells labeling NUB1 using ab171077 at 1/10 dilution (red) or a rabbit IgG negative(green).

      References

      ab171077 has not yet been referenced specifically in any publications.

      Publishing research using ab171077? Please let us know so that we can cite the reference in this datasheet.

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