Recombinant Anti-NUB1 antibody [EPR10716] (ab171077)
![Western blot - Anti-NUB1 antibody [EPR10716] (ab171077)](https://www.abcam.com/ps/products/171/ab171077/Images/ab171077-171862-anti-nyren18-antibody-epr10716-western-blot.jpg "Western blot - Anti-NUB1 antibody [EPR10716] (ab171077)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR10716] to NUB1
- Suitable for: WB, ICC/IF, Flow Cyt
- Reacts with: Human
Overview
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Product name
Anti-NUB1 antibody [EPR10716]
See all NUB1 primary antibodies -
Description
Rabbit monoclonal [EPR10716] to NUB1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, Flow Cytmore details
Unsuitable for: IHC-P or IP -
Species reactivity
Reacts with: Human
Does not react with: Mouse, Rat -
Immunogen
Synthetic peptide within Human NUB1 aa 1-100 (Cysteine residue). The exact sequence is proprietary.
Database link: Q9Y5A7 -
Positive control
- Raji, SHSY5Y, 293T and HeLa cell line lysates. HeLa cells. Permeabilized Raji cells.
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General notes
This product was previously labelled as NYREN18, Nubbin
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR10716 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab171077 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/5000. Predicted molecular weight: 71 kDa. | |
| ICC/IF | 1/100 - 1/250. | |
| Flow Cyt | 1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
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Target
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Function
Specific down-regulator of the NEDD8 conjugation system. Recruits NEDD8, UBD, and their conjugates to the proteasome for degradation. Isoform 1 promotes the degradation of NEDD8 more efficiently than isoform 2. -
Tissue specificity
Widely expressed with lowest expression in the pancreas for isoform 1 and in leukocytes, liver, prostate and skeletal muscle for isoform 2. -
Sequence similarities
Contains 3 UBA domains. -
Cellular localization
Nucleus. Predominantly nuclear. - Information by UniProt
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Database links
- Entrez Gene: 51667 Human
- Omim: 607981 Human
- SwissProt: Q9Y5A7 Human
- Unigene: 647082 Human
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Alternative names
- BS4 antibody
- NEDD8 ultimate buster 1 antibody
- Negative regulator of ubiquitin like proteins 1 antibody
see all
Images
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Western blot - Anti-NUB1 antibody [EPR10716] (ab171077)All lanes : Anti-NUB1 antibody [EPR10716] (ab171077) at 1/1000 dilution
Lane 1 : Raji cell lysate
Lane 2 : SHSY5Y cell lysate
Lane 3 : 293T cell lysate
Lane 4 : HeLa cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 71 kDa -
![Immunocytochemistry/ Immunofluorescence - Anti-NUB1 antibody [EPR10716] (ab171077)](https://www.abcam.com/ps/products/171/ab171077/Images/ab171077-171861-anti-nyren18-antibody-epr10716-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-NUB1 antibody [EPR10716] (ab171077)Immunofluorescent staining of HeLa cells labeling NUB1 using ab171077 at 1/100 dilution.
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![Flow Cytometry - Anti-NUB1 antibody [EPR10716] (ab171077)](https://www.abcam.com/ps/products/171/ab171077/Images/ab171077-171860-anti-nyren18-antibody-epr10716-flow-cytometry.jpg)
Flow Cytometry - Anti-NUB1 antibody [EPR10716] (ab171077)Flow cytometric analysis of permeabilized Raji cells labeling NUB1 using ab171077 at 1/10 dilution (red) or a rabbit IgG negative(green).
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![Anti-NUB1 antibody [EPR10716] (ab171077)](https://www.abcam.com/ps/products/171/ab171077/Images/ab171077-4-benefits-of-recombinant-antibodies.png)
Anti-NUB1 antibody [EPR10716] (ab171077)
Protocols
Datasheets and documents
References (0)
ab171077 has not yet been referenced specifically in any publications.
