Product nameAnti-NUB1 antibody [EPR10717]
See all NUB1 primary antibodies
DescriptionRabbit monoclonal [EPR10717] to NUB1
Tested applicationsSuitable for: Flow Cyt, WBmore details
Unsuitable for: ICC/IF,IHC-P or IP
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human NUB1 aa 400-500. The exact sequence is proprietary.
Database link: Q9Y5A7
- Raji, 293T, HeLa and Jurkat cell lysates; Jurkat cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab178414 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
|WB||1/1000 - 1/5000. Detects a band of approximately 71 kDa (predicted molecular weight: 71 kDa).|
FunctionSpecific down-regulator of the NEDD8 conjugation system. Recruits NEDD8, UBD, and their conjugates to the proteasome for degradation. Isoform 1 promotes the degradation of NEDD8 more efficiently than isoform 2.
Tissue specificityWidely expressed with lowest expression in the pancreas for isoform 1 and in leukocytes, liver, prostate and skeletal muscle for isoform 2.
Sequence similaritiesContains 3 UBA domains.
Cellular localizationNucleus. Predominantly nuclear.
- Information by UniProt
- BS4 antibody
- NEDD8 ultimate buster 1 antibody
- Negative regulator of ubiquitin like proteins 1 antibody
Flow Cytometrical analysis of permeabilized Jurkat cells labeling NYREN18 with ab178414 at 1/10 (red) or a rabbit IgG (negative) (green).
All lanes : Anti-NUB1 antibody [EPR10717] (ab178414) at 1/1000 dilution
Lane 1 : Raji cell lysate
Lane 2 : 293T cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : Jurkat cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 71 kDa
Additional bands at: 71 kDa. We are unsure as to the identity of these extra bands.
ab178414 has not yet been referenced specifically in any publications.