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    nubp1-antibody-ab243521.pdf

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Cell Biology Cell Cycle Cell Division Other cell division antibodies
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Anti-NUBP1 antibody (ab243521)

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Immunocytochemistry/ Immunofluorescence - Anti-NUBP1 antibody (ab243521)
  • Western blot - Anti-NUBP1 antibody (ab243521)
  • Western blot - Anti-NUBP1 antibody (ab243521)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NUBP1 antibody (ab243521)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NUBP1 antibody (ab243521)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NUBP1 antibody (ab243521)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NUBP1 antibody (ab243521)

Key features and details

  • Rabbit polyclonal to NUBP1
  • Suitable for: WB, ICC/IF, IHC-P
  • Reacts with: Human
  • Isotype: IgG

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Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

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Overview

  • Product name

    Anti-NUBP1 antibody
  • Description

    Rabbit polyclonal to NUBP1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IF, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to Human NUBP1 aa 230-320.
    Sequence:

    MSGFICPKCKKESQIFPPTTGGAELMCQDLEVPLLGRVPLDPLIGKNCDK GQSFFIDAPDSPATLAYRSIIQRIQEFCNLHQSKEENLISS


    Database link: P53384
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • IHC-P: Human liver, kidney, testis and colon tissues. WB: Control siRNA transfected U-2 OS cell lysate; RT4 and U-251 MG cell lysates; Human liver and tonsil lysates. ICC/IF: U-2 OS cells.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 40% Glycerol (glycerin, glycerine), PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Cell Cycle
    • Cell Division
    • Other cell division antibodies

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Recombinant Protein

    • Recombinant Human NUBP1 protein (ab202610)
  • Related Products

    • Recombinant Human NUBP1 protein (ab202610)

Applications

Our Abpromise guarantee covers the use of ab243521 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 35 kDa.
ICC/IF Use a concentration of 0.25 - 2 µg/ml.

Fixation/Permeabilization: PFA/Triton X-100.

IHC-P 1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Implicated in the regulation of centrosome duplication (By similarity). Component of the cytosolic iron-sulfur (Fe/S) protein assembly machinery. Required for maturation of extramitochondrial Fe/S proteins. May bind and transfer 2 labile 4Fe-4S clusters to target apoproteins.
  • Sequence similarities

    Belongs to the Mrp/NBP35 ATP-binding proteins family. NUBP1/NBP35 subfamily.
  • Cellular localization

    Cytoplasm.
  • Target information above from: UniProt accession P53384 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 4682 Human
    • Omim: 600280 Human
    • SwissProt: P53384 Human
    • Unigene: 81469 Human
    • Alternative names

      • MGC130052 antibody
      • Cytosolic Fe S cluster assembly factor NUBP1 antibody
      • Cytosolic Fe-S cluster assembly factor NUBP1 antibody
      • MGC117406 antibody
      • MGC130053 antibody
      • NBP 1 antibody
      • NBP antibody
      • NBP1 antibody
      • NBP35 antibody
      • nubp1 antibody
      • NUBP1_HUMAN antibody
      • Nucleotide binding protein (e.coli MinD like) antibody
      • Nucleotide binding protein 1 (E.coli MinD like) antibody
      • Nucleotide binding protein 1 (MinD homolog, E. coli) antibody
      • Nucleotide binding protein 1 antibody
      • Nucleotide-binding protein 1 antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence - Anti-NUBP1 antibody (ab243521)
      Immunocytochemistry/ Immunofluorescence - Anti-NUBP1 antibody (ab243521)

      PFA fixed, Triton X-100 permeabilized U-2 OS (human bone osteosarcoma epithelial cell line) cells labeling NUBP1 using ab243521 at 4 µg/ml (green) in ICC/IF.

    • Western blot - Anti-NUBP1 antibody (ab243521)
      Western blot - Anti-NUBP1 antibody (ab243521)
      All lanes : Anti-NUBP1 antibody (ab243521) at 0.4 µg/ml

      Lane 1 : RT4 (human urinary bladder cancer cell line) cell lysate
      Lane 2 : U-251 MG (human brain glioma cell line) cell lysate
      Lane 3 : Human plasma
      Lane 4 : Human liver lysate
      Lane 5 : Human tonsil lysate

      Predicted band size: 35 kDa

    • Western blot - Anti-NUBP1 antibody (ab243521)
      Western blot - Anti-NUBP1 antibody (ab243521)
      All lanes : Anti-NUBP1 antibody (ab243521) at 0.4 µg/ml

      Lane 1 : siRNA#1 transfected U-2 OS (human bone osteosarcoma epithelial cell line) cell lysate
      Lane 2 : siRNA#2 transfected U-2 OS cell lysate
      Lane 3 : Control siRNA transfected U-2 OS cell lysate

      Predicted band size: 35 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NUBP1 antibody (ab243521)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NUBP1 antibody (ab243521)

      Paraffin-embedded human colon tissue stained for NUBP1 using ab243521 at 1/200 dilution in immunohistochemical analysis.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NUBP1 antibody (ab243521)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NUBP1 antibody (ab243521)

      Paraffin-embedded human kidney tissue stained for NUBP1 using ab243521 at 1/200 dilution in immunohistochemical analysis.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NUBP1 antibody (ab243521)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NUBP1 antibody (ab243521)

      Paraffin-embedded human testis tissue stained for NUBP1 using ab243521 at 1/200 dilution in immunohistochemical analysis.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NUBP1 antibody (ab243521)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NUBP1 antibody (ab243521)

      Paraffin-embedded human liver tissue stained for NUBP1 using ab243521 at 1/200 dilution in immunohistochemical analysis.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (0)

    Publishing research using ab243521? Please let us know so that we can cite the reference in this datasheet.

    ab243521 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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