Anti-NUBPL antibody (ab235924)
Key features and details
- Rabbit polyclonal to NUBPL
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-NUBPL antibody
See all NUBPL primary antibodies -
Description
Rabbit polyclonal to NUBPL -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse -
Immunogen
Recombinant full length protein corresponding to Human NUBPL aa 1 to the C-terminus. Full length mature chain without transit peptide.
Database link: Q8TB37-1 -
Positive control
- WB: A431 whole cell lysate. IHC-P: Human colon cancer and liver cancer tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
Purity >95% -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab235924 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/500 - 1/2000.
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IHC-P |
1/20 - 1/200.
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Notes |
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WB
1/500 - 1/2000. |
IHC-P
1/20 - 1/200. |
Target
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Function
Required for the assembly of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I). May deliver of one or more Fe-S clusters to complex I subunits. -
Tissue specificity
Highest expression in liver and kidney. expressed at significant levels in small intestine and brain (at protein level). -
Involvement in disease
Defects in NUBPL are a cause of mitochondrial complex I deficiency (MT-C1D) [MIM:252010]. A disorder of the mitochondrial respiratory chain that causes a wide range of clinical manifestations from lethal neonatal disease to adult-onset neurodegenerative disorders. Phenotypes include macrocephaly with progressive leukodystrophy, non-specific encephalopathy, cardiomyopathy, myopathy, liver disease, Leigh syndrome, Leber hereditary optic neuropathy, and some forms of Parkinson disease. -
Sequence similarities
Belongs to the Mrp/NBP35 ATP-binding proteins family. -
Cellular localization
Mitochondrion. - Information by UniProt
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Database links
- Entrez Gene: 80224 Human
- Entrez Gene: 76826 Mouse
- Omim: 613621 Human
- SwissProt: Q8TB37 Human
- SwissProt: Q9CWD8 Mouse
- Unigene: 288981 Human
- Unigene: 244781 Mouse
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Alternative names
- C14orf127 antibody
- FLJ12660 antibody
- huInd1 antibody
see all
Images
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Anti-NUBPL antibody (ab235924) at 1/500 dilution + A431 (Human epidermoid carcinoma cell line) whole cell lysate
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution -
Paraffin-embedded human colon cancer tissue stained for NUBPL with ab235924 (1/100 dilution) in immunohistochemical analysis.
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Paraffin-embedded human liver cancer tissue stained for NUBPL with ab235924 (1/100 dilution) in immunohistochemical analysis.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab235924 has not yet been referenced specifically in any publications.