Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11833] to NUBPL
- Suitable for: WB, ICC/IF, IP
- Reacts with: Mouse, Rat, Human
Product nameAnti-NUBPL antibody [EPR11833]
See all NUBPL primary antibodies
DescriptionRabbit monoclonal [EPR11833] to NUBPL
Tested applicationsSuitable for: WB, ICC/IF, IPmore details
Unsuitable for: Flow Cyt or IHC-P
Species reactivityReacts with: Mouse, Rat, Human
Recombinant fragment within Human NUBPL. The exact sequence is proprietary.
Database link: Q8TB37
- Human fetal kidney, 293T cell, HeLa cell and HepG2 cell lysates; HepG2 cells.
Previously labelled as Nucleotide binding protein like.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab171741 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/10000 - 1/50000. Predicted molecular weight: 34 kDa.|
|ICC/IF||1/50 - 1/100.|
|IP||1/10 - 1/100.|
FunctionRequired for the assembly of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I). May deliver of one or more Fe-S clusters to complex I subunits.
Tissue specificityHighest expression in liver and kidney. expressed at significant levels in small intestine and brain (at protein level).
Involvement in diseaseDefects in NUBPL are a cause of mitochondrial complex I deficiency (MT-C1D) [MIM:252010]. A disorder of the mitochondrial respiratory chain that causes a wide range of clinical manifestations from lethal neonatal disease to adult-onset neurodegenerative disorders. Phenotypes include macrocephaly with progressive leukodystrophy, non-specific encephalopathy, cardiomyopathy, myopathy, liver disease, Leigh syndrome, Leber hereditary optic neuropathy, and some forms of Parkinson disease.
Sequence similaritiesBelongs to the Mrp/NBP35 ATP-binding proteins family.
- Information by UniProt
- C14orf127 antibody
- FLJ12660 antibody
- huInd1 antibody
All lanes : Anti-NUBPL antibody [EPR11833] (ab171741) at 1/10000 dilution
Lane 1 : Human fetal kidney lysate
Lane 2 : 293T cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : HepG2 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 34 kDa
Western blot analysis on immunoprecipitation pellet from 293T cell lysate (lane 1) labeling NUBPL, or 1X PBS (Lane 2: negative control) using ab171741 at 1/10 dilution, and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.
Immunofluorescent analysis of HepG2 cells labeling NUBPL using ab171741 at 1/50 dilution.
ab171741 has not yet been referenced specifically in any publications.