Overview

  • Product name

    Anti-NUBPL antibody [EPR11833]
    See all NUBPL primary antibodies
  • Description

    Rabbit monoclonal [EPR11833] to NUBPL
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IF, IPmore details
    Unsuitable for: Flow Cyt or IHC-P
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment within Human NUBPL. The exact sequence is proprietary.
    Database link: Q8TB37

  • Positive control

    • Human fetal kidney, 293T cell, HeLa cell and HepG2 cell lysates; HepG2 cells.
  • General notes

    Previously labelled as Nucleotide binding protein like.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR11833
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab171741 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Predicted molecular weight: 34 kDa.
ICC/IF 1/50 - 1/100.
IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt or IHC-P.
  • Target

    • Function

      Required for the assembly of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I). May deliver of one or more Fe-S clusters to complex I subunits.
    • Tissue specificity

      Highest expression in liver and kidney. expressed at significant levels in small intestine and brain (at protein level).
    • Involvement in disease

      Defects in NUBPL are a cause of mitochondrial complex I deficiency (MT-C1D) [MIM:252010]. A disorder of the mitochondrial respiratory chain that causes a wide range of clinical manifestations from lethal neonatal disease to adult-onset neurodegenerative disorders. Phenotypes include macrocephaly with progressive leukodystrophy, non-specific encephalopathy, cardiomyopathy, myopathy, liver disease, Leigh syndrome, Leber hereditary optic neuropathy, and some forms of Parkinson disease.
    • Sequence similarities

      Belongs to the Mrp/NBP35 ATP-binding proteins family.
    • Cellular localization

      Mitochondrion.
    • Information by UniProt
    • Database links

    • Alternative names

      • C14orf127 antibody
      • FLJ12660 antibody
      • huInd1 antibody
      • IND1 homolog antibody
      • Iron-sulfur protein NUBPL antibody
      • Nubpl antibody
      • NUBPL_HUMAN antibody
      • nucleotide binding protein like antibody
      • Nucleotide-binding protein-like antibody
      see all

    Images

    • All lanes : Anti-NUBPL antibody [EPR11833] (ab171741) at 1/10000 dilution

      Lane 1 : Human fetal kidney lysate
      Lane 2 : 293T cell lysate
      Lane 3 : HeLa cell lysate
      Lane 4 : HepG2 cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 34 kDa

    • Western blot analysis on immunoprecipitation pellet from 293T cell lysate (lane 1) labeling NUBPL, or 1X PBS (Lane 2: negative control) using ab171741 at 1/10 dilution, and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

    • Immunofluorescent analysis of HepG2 cells labeling NUBPL using ab171741 at 1/50 dilution.

    References

    ab171741 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab171741.
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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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