Overview

  • Product name

  • Description

    Rabbit polyclonal to NUCB1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Recombinant fragment corresponding to Human NUCB1 aa 224-461.
    Database link: Q02818

  • Positive control

    • A549, HeLa, HepG2 and Mouse brain whole cell lysates; HeLa cells; Human NCIN87 xenograft tissue.
  • General notes

    Previously labelled as Nucleobindin 1. 

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab154262 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 54 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/1000.

Target

  • Function

    Major calcium-binding protein of the Golgi. May have a role in calcium homeostasis.
  • Tissue specificity

    Expressed both in fetal and adult heart, lung, liver, kidney and brain, and in adult skeletal muscle, placenta and pancreas.
  • Sequence similarities

    Belongs to the nucleobindin family.
    Contains 2 EF-hand domains.
  • Domain

    The EF-hand domains are unfolded in the absence of Ca(2+) and fold upon Ca(2+) addition.
  • Post-translational
    modifications

    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization

    Golgi apparatus > cis-Golgi network membrane. Cytoplasm. A small fraction of the protein may be cytoplasmic.
  • Information by UniProt
  • Database links

  • Alternative names

    • CALNUC antibody
    • FLJ40471 antibody
    • NUC antibody
    • NUCB 1 antibody
    • NUCB1 antibody
    • NUCB1_HUMAN antibody
    • Nucleobindin 1 antibody
    • Nucleobindin-1 antibody
    see all

Images

  • Anti-NUCB1 antibody (ab154262) at 1/1000 dilution + HeLa whole cell lysate at 30 µg

    Predicted band size: 54 kDa



    10% SDS PAGE
  • Anti-NUCB1 antibody (ab154262) at 1/1000 dilution + Mouse brain whole cell lysate at 50 µg

    Predicted band size: 54 kDa



    10% SDS PAGE
  • Immunohistochemical analysis of paraffin-embedded Human NCIN87 xenograft tissue labeling NUCB1 with ab154262 at 1/500 dilution.

  • Immunofluorescent analysis of methanol-fixed HeLa cells labeling NUCB1 with ab154262 at 1/200 dilution. Lower image shows cells co-stained with Hoechst 33342.

References

ab154262 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab154262.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up