Overview

  • Product name

  • Description

    Rabbit polyclonal to NUCB1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Chimpanzee, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide within Human NUCB1 aa 411-461. The exact sequence is proprietary.
    Sequence:

    APAAHPEGQL KFHPDTDDVP VPAPAGDQKE VDTSEKKLLE RLPEVEVPQH L


    Database link: Q02818

  • Positive control

    • HeLa and 293T whole cell lysates.
  • General notes

    Previously labelled as Nucleobindin 1. 

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituent: 99% Tris citrate/phosphate

    pH: 7 to 8
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab206697 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000.
IP Use at 2-10 µg/mg of lysate.

Target

  • Function

    Major calcium-binding protein of the Golgi. May have a role in calcium homeostasis.
  • Tissue specificity

    Expressed both in fetal and adult heart, lung, liver, kidney and brain, and in adult skeletal muscle, placenta and pancreas.
  • Sequence similarities

    Belongs to the nucleobindin family.
    Contains 2 EF-hand domains.
  • Domain

    The EF-hand domains are unfolded in the absence of Ca(2+) and fold upon Ca(2+) addition.
  • Post-translational
    modifications

    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization

    Golgi apparatus > cis-Golgi network membrane. Cytoplasm. A small fraction of the protein may be cytoplasmic.
  • Information by UniProt
  • Database links

  • Alternative names

    • CALNUC antibody
    • FLJ40471 antibody
    • NUC antibody
    • NUCB 1 antibody
    • NUCB1 antibody
    • NUCB1_HUMAN antibody
    • Nucleobindin 1 antibody
    • Nucleobindin-1 antibody
    see all

Images

  • All lanes : Anti-NUCB1 antibody (ab206697) at 0.1 µg/ml

    Lane 1 : HeLa whole cell lysate in NETN lysis buffer
    Lane 2 : 293T whole cell lysate in NETN lysis buffer
    Lane 3 : Jurkat whole cell lysate in NETN lysis buffer

    Lysates/proteins at 50 µg per lane.

    Developed using the ECL technique.

    Exposure time: 30 seconds
  • Immunoprecipitation analysis of 293T cell lysate (in NETN buffer, 0.5 or 1.0 mg per IP reaction; 20% of IP loaded) labeling NUCB1
    using ab206697 at 6 μg per reaction (lane 1). A Control IgG was used in lane 2. For blotting immunoprecipitated NUCB, ab206697 was used at 1 μg/mL. Detection: Chemiluminescence with an exposure time of 30 seconds.

References

ab206697 has not yet been referenced specifically in any publications.

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