Key features and details
- Rabbit polyclonal to NUCB1
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-NUCB1 antibody
See all NUCB1 primary antibodies
DescriptionRabbit polyclonal to NUCB1
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Chimpanzee, Gorilla, Orangutan
- HeLa and 293T whole cell lysates.
Previously labelled as Nucleobindin 1.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH: 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab206697 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000.|
|IP||Use at 2-10 µg/mg of lysate.|
FunctionMajor calcium-binding protein of the Golgi. May have a role in calcium homeostasis.
Tissue specificityExpressed both in fetal and adult heart, lung, liver, kidney and brain, and in adult skeletal muscle, placenta and pancreas.
Sequence similaritiesBelongs to the nucleobindin family.
Contains 2 EF-hand domains.
DomainThe EF-hand domains are unfolded in the absence of Ca(2+) and fold upon Ca(2+) addition.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localizationGolgi apparatus > cis-Golgi network membrane. Cytoplasm. A small fraction of the protein may be cytoplasmic.
- Information by UniProt
- CALNUC antibody
- FLJ40471 antibody
- NUC antibody
All lanes : Anti-NUCB1 antibody (ab206697) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate in NETN lysis buffer
Lane 2 : 293T whole cell lysate in NETN lysis buffer
Lane 3 : Jurkat whole cell lysate in NETN lysis buffer
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Exposure time: 30 seconds
Immunoprecipitation analysis of 293T cell lysate (in NETN buffer, 0.5 or 1.0 mg per IP reaction; 20% of IP loaded) labeling NUCB1
using ab206697 at 6 μg per reaction (lane 1). A Control IgG was used in lane 2. For blotting immunoprecipitated NUCB, ab206697 was used at 1 μg/mL. Detection: Chemiluminescence with an exposure time of 30 seconds.
ab206697 has not yet been referenced specifically in any publications.