Recombinant Anti-NUCB1 antibody [EPR7953] (ab151705)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-NUCB1 antibody [EPR7953]
See all NUCB1 primary antibodies -
Description
Rabbit monoclonal [EPR7953] to NUCB1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details
Unsuitable for: Flow Cyt,ICC or IHC-P -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human NUCB1. The exact sequence is proprietary.
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Positive control
- Human fetal lung, Human fetal heart and HeLa lysates.
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Previously labelled as Nucleobindin 1.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
This product is a recombinant rabbit monoclonal antibody.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.5% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR7953 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab151705 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Predicted molecular weight: 54 kDa. | |
| IP | 1/10 - 1/100. |
Target
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Function
Major calcium-binding protein of the Golgi. May have a role in calcium homeostasis. -
Tissue specificity
Expressed both in fetal and adult heart, lung, liver, kidney and brain, and in adult skeletal muscle, placenta and pancreas. -
Sequence similarities
Belongs to the nucleobindin family.
Contains 2 EF-hand domains. -
Domain
The EF-hand domains are unfolded in the absence of Ca(2+) and fold upon Ca(2+) addition. -
Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localization
Golgi apparatus > cis-Golgi network membrane. Cytoplasm. A small fraction of the protein may be cytoplasmic. - Information by UniProt
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Database links
- Entrez Gene: 4924 Human
- Omim: 601323 Human
- SwissProt: Q02818 Human
- Unigene: 631602 Human
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Alternative names
- CALNUC antibody
- FLJ40471 antibody
- NUC antibody
see all
Images
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![Western blot - Anti-NUCB1 antibody [EPR7953] (ab151705)](https://www.abcam.com/ps/products/151/ab151705/Images/Nucleobindin-1-Primary-antibodies-ab151705-1.jpg)
Western blot - Anti-NUCB1 antibody [EPR7953] (ab151705)All lanes : Anti-NUCB1 antibody [EPR7953] (ab151705) at 1/1000 dilution
Lane 1 : Human fetal lung tissue lysate
Lane 2 : HeLa cell lysate
Lane 3 : Human fetal heart tissue lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 54 kDa
Datasheets and documents
References
ab151705 has not yet been referenced specifically in any publications.
