Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7953] to NUCB1 - BSA and Azide free
- Suitable for: WB, IP
- Reacts with: Human
Product nameAnti-NUCB1 antibody [EPR7953] - BSA and Azide free
See all NUCB1 primary antibodies
DescriptionRabbit monoclonal [EPR7953] to NUCB1 - BSA and Azide free
Tested applicationsSuitable for: WB, IPmore details
Unsuitable for: Flow Cyt,ICC or IHC-P
Species reactivityReacts with: Human
Synthetic peptide within Human NUCB1. The exact sequence is proprietary.
ab249026 is the carrier-free version of ab151705 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Ab249026 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C. Do Not Freeze.
Storage bufferpH: 7.2
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab249026 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration. Predicted molecular weight: 54 kDa.|
|IP||Use at an assay dependent concentration.|
FunctionMajor calcium-binding protein of the Golgi. May have a role in calcium homeostasis.
Tissue specificityExpressed both in fetal and adult heart, lung, liver, kidney and brain, and in adult skeletal muscle, placenta and pancreas.
Sequence similaritiesBelongs to the nucleobindin family.
Contains 2 EF-hand domains.
DomainThe EF-hand domains are unfolded in the absence of Ca(2+) and fold upon Ca(2+) addition.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localizationGolgi apparatus > cis-Golgi network membrane. Cytoplasm. A small fraction of the protein may be cytoplasmic.
- Information by UniProt
- CALNUC antibody
- FLJ40471 antibody
- NUC antibody
All lanes : Anti-NUCB1 antibody [EPR7953] (ab151705) at 1/1000 dilution
Lane 1 : Human fetal lung tissue lysate
Lane 2 : HeLa cell lysate
Lane 3 : Human fetal heart tissue lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 54 kDa
This data was developed using ab151705, the same antibody clone in a different buffer formulation.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab249026 has not yet been referenced specifically in any publications.