Key features and details
- Rabbit polyclonal to NUCB2
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-NUCB2 antibody
See all NUCB2 primary antibodies
DescriptionRabbit polyclonal to NUCB2
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Rat, Human
- WB: Rat brain and heart lysates; Mouse heart, liver and brain lysates. IHC-P: Human prostate cancer tissue.
Previously labelled as Nucleobindin 2.
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We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituents: 50% Glycerol (glycerin, glycerine), PBS, 0.03% Proclin 300
Concentration information loading...
PurityProtein G purified
Purification notesPurity >95%.
Our Abpromise guarantee covers the use of ab223123 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/5000. Detects a band of approximately 51 kDa (predicted molecular weight: 50 kDa).|
|IHC-P||1/20 - 1/200.|
FunctionCalcium-binding protein. May have a role in calcium homeostasis.
Tissue specificityPredominantly expressed in spleen, testis and normal stomach.
Sequence similaritiesBelongs to the nucleobindin family.
Contains 2 EF-hand domains.
Cellular localizationMembrane. Cytoplasm. Secreted.
- Information by UniProt
- DNA binding protein NEFA antibody
- DNA-binding protein NEFA antibody
- Gastric cancer antigen Zg4 antibody
Paraffin-embedded human prostate cancer tissue stained for NUCB2 using ab223123 at 1/100 dilution in immunohistochemical analysis.
All lanes : Anti-NUCB2 antibody (ab223123) at 1/500 dilution
Lane 1 : Rat brain lysate
Lane 2 : Rat heart lysate
Lane 3 : Mouse heart lysate
Lane 4 : Mouse liver lysate
Lane 5 : Mouse brain lysate
All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 50 kDa
Observed band size: 51 kDa why is the actual band size different from the predicted?
ab223123 has not yet been referenced specifically in any publications.