Anti-NUCB2 antibody (ab223123)
Key features and details
- Rabbit polyclonal to NUCB2
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-NUCB2 antibody
See all NUCB2 primary antibodies -
Description
Rabbit polyclonal to NUCB2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment corresponding to Human NUCB2 aa 1-150.
Database link: P80303 -
Positive control
- WB: Rat brain and heart lysates; Mouse heart, liver and brain lysates. IHC-P: Human prostate cancer tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
Purity >95%. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab223123 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/5000. Detects a band of approximately 51 kDa (predicted molecular weight: 50 kDa).
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IHC-P |
1/20 - 1/200.
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Notes |
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WB
1/500 - 1/5000. Detects a band of approximately 51 kDa (predicted molecular weight: 50 kDa). |
IHC-P
1/20 - 1/200. |
Target
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Function
Calcium-binding protein. May have a role in calcium homeostasis. -
Tissue specificity
Predominantly expressed in spleen, testis and normal stomach. -
Sequence similarities
Belongs to the nucleobindin family.
Contains 2 EF-hand domains. -
Cellular localization
Membrane. Cytoplasm. Secreted. - Information by UniProt
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Database links
- Entrez Gene: 4925 Human
- Entrez Gene: 53322 Mouse
- Entrez Gene: 59295 Rat
- Omim: 608020 Human
- SwissProt: P80303 Human
- SwissProt: P81117 Mouse
- SwissProt: Q9JI85 Rat
- Unigene: 654599 Human
see all -
Alternative names
- DNA binding protein NEFA antibody
- DNA-binding protein NEFA antibody
- Gastric cancer antigen Zg4 antibody
see all
Images
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Paraffin-embedded human prostate cancer tissue stained for NUCB2 using ab223123 at 1/100 dilution in immunohistochemical analysis.
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All lanes : Anti-NUCB2 antibody (ab223123) at 1/500 dilution
Lane 1 : Rat brain lysate
Lane 2 : Rat heart lysate
Lane 3 : Mouse heart lysate
Lane 4 : Mouse liver lysate
Lane 5 : Mouse brain lysate
Secondary
All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 50 kDa
Observed band size: 51 kDa why is the actual band size different from the predicted?
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab223123 has not yet been referenced specifically in any publications.