Overview

  • Product name

  • Description

    Rabbit polyclonal to NUCB2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IPmore details
    Unsuitable for: WB
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human NUCB2 aa 175-225. The exact sequence is proprietary.
    Database link: P80303

  • Positive control

    • IP: NUCB2 IP in Jurkat whole cell lysate.
  • General notes

    ab245608 has not performed satisfactorily when used for WB of NUCB2 in crude preparations (e.g. whole cell lysate). This antibody can be used for WB of enriched (e.g. immunoprecipitated) sources of NUCB2.

Properties

Applications

Our Abpromise guarantee covers the use of ab245608 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at 2-10 µg/mg of lysate.
  • Application notes
    Is unsuitable for WB.
  • Target

    • Function

      Calcium-binding protein. May have a role in calcium homeostasis.
    • Tissue specificity

      Predominantly expressed in spleen, testis and normal stomach.
    • Sequence similarities

      Belongs to the nucleobindin family.
      Contains 2 EF-hand domains.
    • Cellular localization

      Membrane. Cytoplasm. Secreted.
    • Information by UniProt
    • Database links

    • Alternative names

      • DNA binding protein NEFA antibody
      • DNA-binding protein NEFA antibody
      • Gastric cancer antigen Zg4 antibody
      • HEL S 109 antibody
      • NEFA antibody
      • Nefastin 1 antibody
      • Nefastin-1 antibody
      • Nefastin1 antibody
      • Nucb2 antibody
      • NUCB2_HUMAN antibody
      • Nucleobindin 2 antibody
      • Nucleobindin-2 antibody
      • Nucleobinding 2 antibody
      • Prepronefastin antibody
      • Prepronesfatin antibody
      see all

    Images

    • NUCB2 was immunoprecipitated from Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate (1 mg for IP, 20% of IP loaded) using ab245608 at 6 μg/reaction. Western blot was performed on the immunoprecipitate using ab245608 at 1 μg/ml.
       
      Lane 1: ab245608 IP in Jurkat whole cell lysate.
      Lane 2: Control IgG in Jurkat whole cell lysate.

      Detection: Chemiluminescence with an exposure time of 10 seconds.

    References

    ab245608 has not yet been referenced specifically in any publications.

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