Key features and details
- Rabbit polyclonal to NUCB2
- Suitable for: IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-NUCB2 antibody
See all NUCB2 primary antibodies
DescriptionRabbit polyclonal to NUCB2
Tested applicationsSuitable for: IPmore details
Species reactivityReacts with: Human
Synthetic peptide within Human NUCB2 aa 175-225. The exact sequence is proprietary.
Database link: P80303
- IP: NUCB2 IP in Jurkat whole cell lysate.
ab245608 has not performed satisfactorily when used for WB of NUCB2 in crude preparations (e.g. whole cell lysate). This antibody can be used for WB of enriched (e.g. immunoprecipitated) sources of NUCB2.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab245608 was affinity purified using an epitope specific to NUCB2 immobilized on solid support.
Our Abpromise guarantee covers the use of ab245608 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at 2-10 µg/mg of lysate.|
FunctionCalcium-binding protein. May have a role in calcium homeostasis.
Tissue specificityPredominantly expressed in spleen, testis and normal stomach.
Sequence similaritiesBelongs to the nucleobindin family.
Contains 2 EF-hand domains.
Cellular localizationMembrane. Cytoplasm. Secreted.
- Information by UniProt
- DNA binding protein NEFA antibody
- DNA-binding protein NEFA antibody
- Gastric cancer antigen Zg4 antibody
NUCB2 was immunoprecipitated from Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate (1 mg for IP, 20% of IP loaded) using ab245608 at 6 μg/reaction. Western blot was performed on the immunoprecipitate using ab245608 at 1 μg/ml.
Lane 1: ab245608 IP in Jurkat whole cell lysate.
Lane 2: Control IgG in Jurkat whole cell lysate.
Detection: Chemiluminescence with an exposure time of 10 seconds.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab245608 has not yet been referenced specifically in any publications.