Product nameAnti-NUCB2 antibody - C-terminal
See all NUCB2 primary antibodies
DescriptionRabbit polyclonal to NUCB2 - C-terminal
Tested applicationsSuitable for: IHC-P, WB, ICC/IF, IPmore details
Species reactivityReacts with: Rat, Human
Recombinant fragment within Human NUCB2 (C terminal). The exact sequence is proprietary.
Database link: P80303
- WB: HEK-293T, A431, HeLa and HepG2 whole cell extracts. IP: HEK-293T whole cell extract. IHC-P: Rat brain tissue. ICC/IF: A431 cells.
Previously labelled as Nucleobindin 2.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.025% Proclin
Constituents: PBS, 20% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab229683 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/100 - 1/1000.|
|WB||1/500 - 1/3000. Predicted molecular weight: 50 kDa.|
|ICC/IF||1/100 - 1/1000.|
|IP||1/100 - 1/500.|
FunctionCalcium-binding protein. May have a role in calcium homeostasis.
Tissue specificityPredominantly expressed in spleen, testis and normal stomach.
Sequence similaritiesBelongs to the nucleobindin family.
Contains 2 EF-hand domains.
Cellular localizationMembrane. Cytoplasm. Secreted.
- Information by UniProt
- DNA binding protein NEFA antibody
- DNA-binding protein NEFA antibody
- Gastric cancer antigen Zg4 antibody
Paraffin-embedded rat brain tissue stained for NUCB2 using ab229683 at 1/500 dilution in immunohistochemical analysis.
All lanes : Anti-NUCB2 antibody - C-terminal (ab229683) at 1/1000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell extract
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 50 kDa
10% SDS-PAGE gel.
4% paraformaldehyde-fixed A431 (human epidermoid carcinoma cell line) cells stained for NUCB2 (green) using ab229683 at 1/500 dilution in ICC/IF.
Nuclear counterstain: Hoechst 33342 (blue).
NUCB2 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract with 5 µg of ab229683. Western blot was performed from the immunoprecipitate using ab229683. Anti-Rabbit IgG was used as a secondary reagent.
Lane 1: HEK-293T whole cell extract (Input).
Lane 2: Control IgG instead of ab229683 in HEK-293T whole cell extract.
Lane 3: ab229683 IP in HEK-293T whole cell extract.