Key features and details
- Rabbit polyclonal to NUCB2 - C-terminal
- Suitable for: IHC-P, WB, ICC/IF, IP
- Reacts with: Rat, Human
- Isotype: IgG
Product nameAnti-NUCB2 antibody - C-terminal
See all NUCB2 primary antibodies
DescriptionRabbit polyclonal to NUCB2 - C-terminal
Tested applicationsSuitable for: IHC-P, WB, ICC/IF, IPmore details
Species reactivityReacts with: Rat, Human
Recombinant fragment within Human NUCB2 (C terminal). The exact sequence is proprietary.
Database link: P80303
- WB: HEK-293T, A431, HeLa and HepG2 whole cell extracts. IP: HEK-293T whole cell extract. IHC-P: Rat brain tissue. ICC/IF: A431 cells.
Previously labelled as Nucleobindin 2.
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We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab229683 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/100 - 1/1000.|
|WB||1/500 - 1/3000. Predicted molecular weight: 50 kDa.|
|ICC/IF||1/100 - 1/1000.|
|IP||1/100 - 1/500.|
FunctionCalcium-binding protein. May have a role in calcium homeostasis.
Tissue specificityPredominantly expressed in spleen, testis and normal stomach.
Sequence similaritiesBelongs to the nucleobindin family.
Contains 2 EF-hand domains.
Cellular localizationMembrane. Cytoplasm. Secreted.
- Information by UniProt
- DNA binding protein NEFA antibody
- DNA-binding protein NEFA antibody
- Gastric cancer antigen Zg4 antibody
Paraffin-embedded rat brain tissue stained for NUCB2 using ab229683 at 1/500 dilution in immunohistochemical analysis.
All lanes : Anti-NUCB2 antibody - C-terminal (ab229683) at 1/1000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell extract
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 50 kDa
10% SDS-PAGE gel.
4% paraformaldehyde-fixed A431 (human epidermoid carcinoma cell line) cells stained for NUCB2 (green) using ab229683 at 1/500 dilution in ICC/IF.
Nuclear counterstain: Hoechst 33342 (blue).
NUCB2 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract with 5 µg of ab229683. Western blot was performed from the immunoprecipitate using ab229683. Anti-Rabbit IgG was used as a secondary reagent.
Lane 1: HEK-293T whole cell extract (Input).
Lane 2: Control IgG instead of ab229683 in HEK-293T whole cell extract.
Lane 3: ab229683 IP in HEK-293T whole cell extract.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab229683 has been referenced in 2 publications.
- Liu QJ et al. Nucleobindin-2 Promotes the Growth and Invasion of Glioblastoma. Cancer Biother Radiopharm 34:581-588 (2019). PubMed: 31697592
- Zhang D et al. Regulation of the adaptation to ER stress by KLF4 facilitates melanoma cell metastasis via upregulating NUCB2 expression. J Exp Clin Cancer Res 37:176 (2018). PubMed: 30055641