Overview

  • Product name

    Anti-NUCB2 antibody - C-terminal
    See all NUCB2 primary antibodies
  • Description

    Rabbit polyclonal to NUCB2 - C-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WB, ICC/IF, IPmore details
  • Species reactivity

    Reacts with: Rat, Human
  • Immunogen

    Recombinant fragment within Human NUCB2 (C terminal). The exact sequence is proprietary.
    Database link: P80303

  • Positive control

    • WB: HEK-293T, A431, HeLa and HepG2 whole cell extracts. IP: HEK-293T whole cell extract. IHC-P: Rat brain tissue. ICC/IF: A431 cells.
  • General notes

    Previously labelled as Nucleobindin 2. 

Properties

Applications

Our Abpromise guarantee covers the use of ab229683 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/1000.
WB 1/500 - 1/3000. Predicted molecular weight: 50 kDa.
ICC/IF 1/100 - 1/1000.
IP 1/100 - 1/500.

Target

  • Function

    Calcium-binding protein. May have a role in calcium homeostasis.
  • Tissue specificity

    Predominantly expressed in spleen, testis and normal stomach.
  • Sequence similarities

    Belongs to the nucleobindin family.
    Contains 2 EF-hand domains.
  • Cellular localization

    Membrane. Cytoplasm. Secreted.
  • Information by UniProt
  • Database links

  • Alternative names

    • DNA binding protein NEFA antibody
    • DNA-binding protein NEFA antibody
    • Gastric cancer antigen Zg4 antibody
    • HEL S 109 antibody
    • NEFA antibody
    • Nefastin 1 antibody
    • Nefastin-1 antibody
    • Nefastin1 antibody
    • Nucb2 antibody
    • NUCB2_HUMAN antibody
    • Nucleobindin 2 antibody
    • Nucleobindin-2 antibody
    • Nucleobinding 2 antibody
    • Prepronefastin antibody
    • Prepronesfatin antibody
    see all

Images

  • Paraffin-embedded rat brain tissue stained for NUCB2 using ab229683 at 1/500 dilution in immunohistochemical analysis.

  • All lanes : Anti-NUCB2 antibody - C-terminal (ab229683) at 1/1000 dilution

    Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
    Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell extract
    Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
    Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 50 kDa



    10% SDS-PAGE gel.

  • 4% paraformaldehyde-fixed A431 (human epidermoid carcinoma cell line) cells stained for NUCB2 (green) using ab229683 at 1/500 dilution in ICC/IF.

    Nuclear counterstain: Hoechst 33342 (blue).

  • NUCB2 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract with 5 µg of ab229683. Western blot was performed from the immunoprecipitate using ab229683. Anti-Rabbit IgG was used as a secondary reagent.

    Lane 1: HEK-293T whole cell extract (Input). 
    Lane 2: Control IgG instead of ab229683 in HEK-293T whole cell extract. 
    Lane 3: ab229683 IP in HEK-293T whole cell extract. 

References

This product has been referenced in:

  • Zhang D  et al. Regulation of the adaptation to ER stress by KLF4 facilitates melanoma cell metastasis via upregulating NUCB2 expression. J Exp Clin Cancer Res 37:176 (2018). Read more (PubMed: 30055641) »
See 1 Publication for this product

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