Overview

  • Product name

  • Description

    Rabbit polyclonal to NUCKS1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Horse, Guinea pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide, corresponding to a region within residues 1 and 50 of Human NUCKS1, NP_073568.2

  • Positive control

    • Human breast carcinoma, prostate carcinoma, Mouse renal cell carcinoma

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab84710 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
  • Application notes
    IHC-P: 1/100 - 1/500. Epitope exposure is recommended. Epitope exposure with citrate buffer will enhance staining.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Post-translational
      modifications

      Phosphorylated by CDK1 and casein kinase. Phosphorylated upon DNA damage, probably by ATM or ATR.
    • Cellular localization

      Nucleus.
    • Information by UniProt
    • Database links

    • Alternative names

      • FLJ21480 antibody
      • FLJ32016 antibody
      • FLJ38536 antibody
      • JC7 antibody
      • NUCKS 1 antibody
      • NUCKS antibody
      • NUCKS_HUMAN antibody
      • Nucks1 antibody
      • Nuclear casein kinase and cyclin dependent kinase substrate 1 antibody
      • Nuclear ubiquitous casein and cyclin dependent kinases substrate antibody
      • Nuclear ubiquitous casein and cyclin-dependent kinases substrate antibody
      • Nuclear ubiquitous casein kinase and cyclin dependent kinase substrate antibody
      • P1 antibody
      • Potential LAG1 interactor antibody
      see all

    Images

    • Detection of Human NUCKS1 by Immunofluorescence.
      Sample: formalin fixed, paraffin embedded section of Human breast carcinoma.
      Antibody: ab84710 used at a dilution of 1/100.
      Detection: Red-fluorescent goat anti-rabbit IgG highly cross-adsorbed Antibody Hilyte PlusTM 555 used at a dilution of 1/100.
    • Detection of Human NUCKS1 by Immunohistochemistry.
      Sample: FFPE section of Human prostate carcinoma.
      Antibody: ab84710 used at a dilution of 1/250.
      Detection: DAB staining.
    • Detection of Mouse NUCKS1 by Immunohistochemistry.
      Sample: FFPE section of Mouse renal cell carcinoma.
      Antibody: ab84710 used at a dilution of 1/250.
      Detection: DAB staining.

    References

    This product has been referenced in:

    • Gupta MK  et al. Altered transcriptional regulatory proteins in glioblastoma and YBX1 as a potential regulator of tumor invasion. Sci Rep 9:10986 (2019). Read more (PubMed: 31358880) »
    • Kikuchi A  et al. Identification of NUCKS1 as a colorectal cancer prognostic marker through integrated expression and copy number analysis. Int J Cancer : (2012). IHC-P ; Human . Read more (PubMed: 23065711) »
    See all 2 Publications for this product

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