Nuclear Extraction Kit (ab113474)
Key features and details
- Assay time: 1 hr
- Sample type: Adherent cells, Suspension cells, Tissue
Overview
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Product name
Nuclear Extraction Kit
See all Nuclear Extraction kits -
Sample type
Tissue, Adherent cells, Suspension cells -
Assay time
1h 00m -
Product overview
Nuclear Extraction Kit (ab113474) provides a simple and selective method along with all necessary reagents for nuclear protein extraction / nuclear protein fractionation in just 1 hour.
The extracts can then be used in western blotting, protein-DNA binding assays, nuclear enzyme assays or any other procedures requiring optimized nuclear proteins. The protocol is fast and easy-to-use, and isolates very abundant yields of nuclear extract from mammalian cells or tissue samples.
Not sure if this is the right product for you? Check out our EpiSeeker Sample Preparation Guide for help.
Compared to other kits that use conventional nuclear extraction / nuclear fractionation methods, the buffers included in ab113474 contain much lower amounts of salts (80% less than conventional kits) and no SDS, which allows much better retention of enzyme activity in the nuclear extracts.
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 100 tests 1000X Protease Inhibitor Cocktail 1 x 110µl 10X Pre-Extraction Buffer 1 x 10ml DTT Solution (1000X) 1 x 110µl ENE2 (Extraction Buffer) 1 x 10ml -
Research areas
Associated products
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Related Products
Images
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Functional Studies - Nuclear Extraction Kit (ab113474)Ragab, Diaa et al., PloS one?vol. 9,5 e95313., Fig 6, doi:10.1371/journal.pone.0095313
Effect of two drugs refered to as Cilo (50 and 100 mg/kg; Cilo50and Cilo100), and Pio (3 and 10 mg/kg; Pio3 and Pio10), and their combination (Cilo50and Pio3) on the PPAR-γ transcription activity in rats subjected to ischemia (45 min)/reperfusion (24 hrs).
Drugs were administered orally for 14 days then subjected to ischemia/reperfusion. Values are expressed as mean ± S.E.M (n = 6). Data are compared with sham operated control (#), I/R control (∗), Cilo50 (), Pio3 (), and combination (§) pretreated groups (one-way ANOVA followed by Tukey Multiple Comparison Test) at P<0.05. -
Functional Studies - Nuclear Extraction Kit (ab113474)Park, Jisu et al., PloS one?vol. 10,11 e0141988., Fig 4, doi:10.1371/journal.pone.0141988B16F10 cells were treated with 30 µM of DMPB for the indicated time periods. Cytoplasmic and nuclear fractions were isolated and analyzed by Western blotting. -
Functional Studies - Nuclear Extraction Kit (ab113474)Nuclear extracts were prepared from MCF-7 cells and the activity of HDACs were measured using different amounts of the extract. The result shown in the figure demonstrates the ab113474's high specificity.
Datasheets and documents
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SDS download
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Datasheet download
References (150)
ab113474 has been referenced in 150 publications.
- Zarei M et al. Ginger and turmeric lipid-solubles attenuate heated oil-induced oxidative stress in the brain via the upregulation of NRF2 and improve cognitive function in rats. Metab Brain Dis 36:225-238 (2021). PubMed: 33170419
- Aguiari P et al. Persistent COUP-TFII expression underlies the myopathy and impaired muscle regeneration observed in resistance to thyroid hormone-alpha. Sci Rep 11:4601 (2021). PubMed: 33633251
- Hu R et al. Lactobacillus reuteri-derived extracellular vesicles maintain intestinal immune homeostasis against lipopolysaccharide-induced inflammatory responses in broilers. J Anim Sci Biotechnol 12:25 (2021). PubMed: 33593426
- Kapetanou M et al. FoxO1 Is a Novel Regulator of 20S Proteasome Subunits Expression and Activity. Front Cell Dev Biol 9:625715 (2021). PubMed: 33634126
- Li L et al. Sunitinib treatment promotes metastasis of drug-resistant renal cell carcinoma via TFE3 signaling pathway. Cell Death Dis 12:220 (2021). PubMed: 33637706