Question (76723) | Nuclear Extraction Kit (ab113474)

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Question


Since we are concern about the nuclear integrity, we do not care too much about the cytosol. Therefore, DTT may can be excluded in Preextraction buffer too, because its main effect is to avoid the oxidation of the protein. Am I right?

Answer



I can confirm that if the cytosol is not required for much consideration, DTT can be excluded in pre-lysis buffer too.

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