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I'm interested on the Nuclear Extraction Kit (ab113474). I would like to know if have you ever tried it with less amount of cells than what is written on the protocol booklet (around only half million cells).
Moreover, if after the extraction it is possible to quantify the protein amount with the Pierce BCA assay (if any component of the pre-extraction buffer or of the extraction buffer can interfere).
Asked on Oct 03 2016
This kit will work better with 2 million cells however you can still use this with half a million cells, when using with 500,000 cells please keep the volume of buffers and reagents proportional to 1 million cells.
We recommend using Bradford for quantification, but the BCA Pierce assay will also be suitable.
Answered on Oct 03 2016