Nuclear Green DCS1 (ab138905)
- Datasheet
- References (2)
- Protocols
Overview
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Product name
Nuclear Green DCS1 -
Tested applications
Suitable for: ICC/IF, Flow Cytmore details -
General notes
Nuclear Green DCS1 is a fluorogenic, DNA-selective and cell impermeant green fluorescent dye for analyzing DNA content in dead cells. The Nuclear Green DCS1 has its fluorescence significantly enhanced upon binding to double-stranded DNA. It can be used in fluorescence imaging, microplate and flow cytometry applications. This DNA-binding dye might be used for multicolor analysis of dead cells.
SPECTRAL PROPERTIES:
Excitation: Exmax 503 nm
Emission: Emmax 526 nm
Provided at 5mM. Recommended use at 2 - 10 uM.
Properties
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Form
Liquid -
Storage instructions
Store at -20°C. Store In the Dark. -
Concentration information loading... -
Research areas
Applications
Our Abpromise guarantee covers the use of ab138905 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | Use at an assay dependent concentration. | |
| Flow Cyt | Use at an assay dependent concentration. |
Images
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Immunocytochemistry/ Immunofluorescence - Nuclear Green DCS1 (ab138905)ICC/IF image of ab7291 stained HeLa cells. The cells were 100% methanol fixed and then incubated in 1% BSA / 10% normal donkey serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilize the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab7291, 5µg/ml) overnight at +4°C. The secondary antibody (red) was ab150115 Alexa Fluor® 647 goat anti-mouse IgG (H+L) used at 2µg/ml for 1h. Nuclear Green DCS1 (ab138905) was used to stain the cell nuclei (green) at a 1/1500 dilution with a 60 minute incubation.
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Functional Studies - Nuclear Green DCS1 (ab138905)Excitation and emission spectra for ab138905 Nuclear Green DCS1 bound to DNA in PBS (pH 7.4).
Protocols
References
This product has been referenced in:
- Suzuki H et al. Generation of bicistronic reporter knockin mice for visualizing germ layers. Exp Anim 68:499-509 (2019). Read more (PubMed: 31189761) »
- Liu GY et al. Templated Assembly of Collagen Fibers Directs Cell Growth in 2D and 3D. Sci Rep 7:9628 (2017). Read more (PubMed: 28852121) »
