Nuclear Green DCS1 (ab138905)

Submit a review Q&A (1)References (4)
Immunocytochemistry/ Immunofluorescence - Nuclear Green DCS1 (ab138905)
  • Functional Studies - Nuclear Green DCS1 (ab138905)

Overview

  • Product name

    Nuclear Green DCS1

  • Tested applications

    Suitable for: ICC/IF, Flow Cytmore details

  • General notes

    Nuclear Green DCS1 is a fluorogenic, DNA-selective and cell impermeant green fluorescent dye for analyzing DNA content in dead cells. The Nuclear Green DCS1 has its fluorescence significantly enhanced upon binding to double-stranded DNA. It can be used in fluorescence imaging, microplate and flow cytometry applications. This DNA-binding dye might be used for multicolor analysis of dead cells.

    SPECTRAL PROPERTIES:

    Excitation: Exmax 503 nm

    Emission: Emmax 526 nm

    Provided at 5mM. Recommended use at 2 - 10 uM.

Properties

Associated products

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab138905 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    ICC/IF
    Use at an assay dependent concentration.
    Flow Cyt
    Use at an assay dependent concentration.
    Notes
    ICC/IF
    Use at an assay dependent concentration.
    Flow Cyt
    Use at an assay dependent concentration.

    Images

    • Immunocytochemistry/ Immunofluorescence - Nuclear Green DCS1 (ab138905)
      Immunocytochemistry/ Immunofluorescence - Nuclear Green DCS1 (ab138905)

      ICC/IF image of ab7291 stained HeLa cells. The cells were 100% methanol fixed and then incubated in 1% BSA / 10% normal donkey serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilize the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab7291, 5µg/ml) overnight at +4°C. The secondary antibody (red) was ab150115 Alexa Fluor® 647 goat anti-mouse IgG (H+L) used at 2µg/ml for 1h. Nuclear Green DCS1 (ab138905) was used to stain the cell nuclei (green) at a 1/1500 dilution with a 60 minute incubation.

    • Functional Studies - Nuclear Green DCS1 (ab138905)
      Functional Studies - Nuclear Green DCS1 (ab138905)
      Excitation and emission spectra for ab138905 Nuclear Green DCS1 bound to DNA in PBS (pH 7.4).

    References (4)

    Publishing research using ab138905? Please let us know so that we can cite the reference in this datasheet.

    ab138905 has been referenced in 4 publications.

    • Sindi HA  et al. Therapeutic potential of KLF2-induced exosomal microRNAs in pulmonary hypertension. Nat Commun 11:1185 (2020). PubMed: 32132543
    • Suzuki H  et al. Generation of bicistronic reporter knockin mice for visualizing germ layers. Exp Anim 68:499-509 (2019). PubMed: 31189761
    • Aichem A  et al. The ubiquitin-like modifier FAT10 interferes with SUMO activation. Nat Commun 10:4452 (2019). PubMed: 31575873
    • Liu GY  et al. Templated Assembly of Collagen Fibers Directs Cell Growth in 2D and 3D. Sci Rep 7:9628 (2017). PubMed: 28852121

    Customer reviews and Q&As

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    Question





    I would like to know the stock concentration (Molarity) of the specific product. Thank you in advance

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    Answer

    Thank you for getting in touch.



    The stock solution of ab138905 is 5 mM. This should be used at 2 - 10 µM.



    I am happy to answer any further questions you have.

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