Overview

  • Product name

    Nuclear Green DCS1
  • Tested applications

    Suitable for: ICC/IF, Flow Cytmore details
  • General notes

    Nuclear Green DCS1 is a fluorogenic, DNA-selective and cell impermeant green fluorescent dye for analyzing DNA content in dead cells. The Nuclear Green DCS1 has its fluorescence significantly enhanced upon binding to double-stranded DNA. It can be used in fluorescence imaging, microplate and flow cytometry applications. This DNA-binding dye might be used for multicolor analysis of dead cells.

    SPECTRAL PROPERTIES:

    Excitation: Exmax 503 nm

    Emission: Emmax 526 nm

    Provided at 5mM. Recommended use at 2 - 10 uM.

Properties

Applications

Our Abpromise guarantee covers the use of ab138905 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.

Images

  • ICC/IF image of ab7291 stained HeLa cells. The cells were 100% methanol fixed and then incubated in 1% BSA / 10% normal donkey serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilize the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab7291, 5µg/ml) overnight at +4°C. The secondary antibody (red) was ab150115 Alexa Fluor® 647 goat anti-mouse IgG (H+L) used at 2µg/ml for 1h. Nuclear Green DCS1 (ab138905) was used to stain the cell nuclei (green) at a 1/1500 dilution with a 60 minute incubation.

  • Excitation and emission spectra for ab138905 Nuclear Green DCS1 bound to DNA in PBS (pH 7.4).

Protocols

References

This product has been referenced in:

See 1 Publication for this product

Customer reviews and Q&As

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The stock solution of ab138905 is 5 mM. This should be used at 2 - 10 µM.



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