Overview

  • Product name

    Nuclear Green LCS1
  • Tested applications

    Suitable for: ICC/IF, Flow Cytmore details
  • General notes

    Nuclear Green LCS1 is a fluorogenic, DNA-selective and cell-permeant dye for analyzing DNA content in living cells. The Nuclear Green LCS1 has its green fluorescence significantly enhanced upon binding to double-stranded DNA. It can be used in fluorescence imaging, microplate and flow cytometry applications. This DNA-binding dye might be used for multicolor analysis of live and fixed cells.

     

    SPECTRAL PROPERTIES:

    Excitation: Exmax 503 nm

    Emission: Emmax 526 nm

Properties

Applications

Our Abpromise guarantee covers the use of ab138904 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration. Stock solution is 5mM. Working concentration is 2 - 10uM.
Flow Cyt Use at an assay dependent concentration.

Images

  • Excitation and emission spectra for ab138904 Nuclear Green LCS1 bound to DNA in PBS (pH 7.4).

Protocols

References

This product has been referenced in:

  • Kamada R  et al. Effective Cellular Morphology Analysis for Differentiation Processes by a Fluorescent 1,3a,6a-Triazapentalene Derivative Probe in Live Cells. PLoS One 11:e0160625 (2016). Read more (PubMed: 27490470) »
See 1 Publication for this product

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