Nuclear Green LCS1 (ab138904)
Overview
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Product name
Nuclear Green LCS1 -
Tested applications
Suitable for: ICC/IF, Flow Cytmore details -
General notes
Nuclear Green LCS1 is a fluorogenic, DNA-selective and cell-permeant dye for analyzing DNA content in living cells. The Nuclear Green LCS1 has its green fluorescence significantly enhanced upon binding to double-stranded DNA. It can be used in fluorescence imaging, microplate and flow cytometry applications. This DNA-binding dye might be used for multicolor analysis of live and fixed cells.
SPECTRAL PROPERTIES:
Excitation: Exmax 503 nm
Emission: Emmax 526 nm
Properties
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Form
Liquid -
Storage instructions
Store at -20°C. Store In the Dark. -
Storage buffer
Constituent: 99% Dimethylsulfoxide -
Concentration information loading... -
Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab138904 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF |
Use at an assay dependent concentration. Stock solution is 5mM. Working concentration is 2 - 10uM.
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| Flow Cyt |
Use at an assay dependent concentration.
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| Notes |
|---|
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ICC/IF
Use at an assay dependent concentration. Stock solution is 5mM. Working concentration is 2 - 10uM. |
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Flow Cyt
Use at an assay dependent concentration. |
Images
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Functional Studies - Nuclear Green LCS1 (ab138904)Excitation and emission spectra for ab138904 Nuclear Green LCS1 bound to DNA in PBS (pH 7.4).
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab138904 has been referenced in 3 publications.
- Freund EC et al. Efficient gene knockout in primary human and murine myeloid cells by non-viral delivery of CRISPR-Cas9. J Exp Med 217:N/A (2020). PubMed: 32357367
- Huynh N et al. A versatile toolkit for CRISPR-Cas13-based RNA manipulation in Drosophila. Genome Biol 21:279 (2020). PubMed: 33203452
- Kamada R et al. Effective Cellular Morphology Analysis for Differentiation Processes by a Fluorescent 1,3a,6a-Triazapentalene Derivative Probe in Live Cells. PLoS One 11:e0160625 (2016). PubMed: 27490470