Key features and details
- Rabbit polyclonal to Nuclear Matrix Protein p84
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Nuclear Matrix Protein p84 antibody
See all Nuclear Matrix Protein p84 primary antibodies
DescriptionRabbit polyclonal to Nuclear Matrix Protein p84
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
Recombinant protein fragment containing a sequence corresponding to a region within amino acids 469 and 628 of Human Nuclear Matrix Protein p84 (NP_005122).
- A549, HeLa, HepG2 and HCT116 whole cell lysates. NCIN87 xenograft.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab102684 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 76 kDa.|
|IHC-P||1/100 - 1/500. Suggested antigen retrieval using heat mediated 10mM Citrate buffer or Tris-EDTA buffer (PH8.0).|
FunctionComponent of the THO subcomplex of the TREX complex. The TREX complex specifically associates with spliced mRNA and not with unspliced pre-mRNA. It is recruited to spliced mRNAs by a transcription-independent mechanism. Binds to mRNA upstream of the exon-junction complex (EJC) and is recruited in a splicing- and cap-dependent manner to a region near the 5' end of the mRNA where it functions in mRNA export. The recruitment occurs via an interaction between THOC4 and the cap-binding protein NCBP1. DDX39B functions as a bridge between THOC4 and the THO complex. The TREX complex is essential for the export of Kaposi's sarcoma-associated herpesvirus (KSHV) intronless mRNAs and infectious virus production. The recruitment of the TREX complex to the intronless viral mRNA occurs via an interaction between KSHV ORF57 protein and THOC4.
Regulates transcriptional elongation of a subset of genes. Participates in an apoptotic pathway which is characterized by activation of caspase-6, increases in the expression of BAK1 and BCL2L1 and activation of NF-kappa-B. This pathway does not require p53/TP53, nor does the presence of p53/TP53 affect the efficiency of cell killing. Activates a G2/M cell cycle checkpoint prior to the onset of apoptosis. Apoptosis is inhibited by association with RB1.
Tissue specificityUbiquitous. Expressed in various cancer cell lines. Expressed at very low levels in normal breast epithelial cells and highly expressed in breast tumors. Expression is strongly associated with an aggressive phenotype of breast tumors and expression correlates with tumor size and the metastatic state of the tumor progression.
Sequence similaritiesContains 1 death domain.
DomainAn intact death domain is needed for apoptosis.
modificationsExpression is altered specifically during apoptosis and is accompanied by the appearance of novel forms with smaller apparent molecular mass.
Cellular localizationCytoplasm and Nucleus speckle. Nucleus > nucleoplasm. Nucleus matrix. Cytoplasm. Can shuttle between the nucleus and cytoplasm. Nuclear localization is required for induction of apoptotic cell death. Translocates to the cytoplasm during the early phase of apoptosis execution.
- Information by UniProt
FormNuclear (Isoform 1) and Cytoplasmic (Isoform 1 and 2).
- hTREX84 antibody
- Death domain containing protein p84N5 antibody
- HPR 1 antibody
ab102684 has been referenced in 6 publications.
- Jiang L et al. NCAPG confers trastuzumab resistance via activating SRC/STAT3 signaling pathway in HER2-positive breast cancer. Cell Death Dis 11:547 (2020). PubMed: 32683421
- Li H et al. Nucleolar and spindle associated protein 1 promotes metastasis of cervical carcinoma cells by activating Wnt/ß-catenin signaling. J Exp Clin Cancer Res 38:33 (2019). PubMed: 30678687
- Ren D et al. Wnt5a induces and maintains prostate cancer cells dormancy in bone. J Exp Med 216:428-449 (2019). PubMed: 30593464
- Yu X et al. Baicalein induces cervical cancer apoptosis through the NF-?B signaling pathway. Mol Med Rep 17:5088-5094 (2018). PubMed: 29393414
- Li Q et al. PHF21B overexpression promotes cancer stem cell-like traits in prostate cancer cells by activating the Wnt/ß-catenin signaling pathway. J Exp Clin Cancer Res 36:85 (2017). WB . PubMed: 28645312
- Zhang X et al. Phospholipid Phosphatase 4 promotes proliferation and tumorigenesis, and activates Ca2+-permeable Cationic Channel in lung carcinoma cells. Mol Cancer 16:147 (2017). PubMed: 28851360