Anti-Nuclear Matrix Protein p84 antibody (ab245671)
Key features and details
- Rabbit polyclonal to Nuclear Matrix Protein p84
- Suitable for: IP, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Nuclear Matrix Protein p84 antibody
See all Nuclear Matrix Protein p84 primary antibodies -
Description
Rabbit polyclonal to Nuclear Matrix Protein p84 -
Host species
Rabbit -
Tested applications
Suitable for: IP, WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human Nuclear Matrix Protein p84 aa 607-657. The exact sequence is proprietary. NP_005122.2
Database link: Q96FV9 -
Positive control
- IHC-P: Human ovarian carcinoma tissue. WB: HeLa, HEK-293T and Jurkat whole cell lysate. IP: HeLa whole cell lysate.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab245671 was affinity purified using an epitope specific to Nuclear Matrix Protein p84 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab245671 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IP | Use at 2-5 µg/mg of lysate. | |
| WB | 1/2000 - 1/10000. Predicted molecular weight: 76 kDa. | |
| IHC-P | 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Component of the THO subcomplex of the TREX complex. The TREX complex specifically associates with spliced mRNA and not with unspliced pre-mRNA. It is recruited to spliced mRNAs by a transcription-independent mechanism. Binds to mRNA upstream of the exon-junction complex (EJC) and is recruited in a splicing- and cap-dependent manner to a region near the 5' end of the mRNA where it functions in mRNA export. The recruitment occurs via an interaction between THOC4 and the cap-binding protein NCBP1. DDX39B functions as a bridge between THOC4 and the THO complex. The TREX complex is essential for the export of Kaposi's sarcoma-associated herpesvirus (KSHV) intronless mRNAs and infectious virus production. The recruitment of the TREX complex to the intronless viral mRNA occurs via an interaction between KSHV ORF57 protein and THOC4.
Regulates transcriptional elongation of a subset of genes. Participates in an apoptotic pathway which is characterized by activation of caspase-6, increases in the expression of BAK1 and BCL2L1 and activation of NF-kappa-B. This pathway does not require p53/TP53, nor does the presence of p53/TP53 affect the efficiency of cell killing. Activates a G2/M cell cycle checkpoint prior to the onset of apoptosis. Apoptosis is inhibited by association with RB1. -
Tissue specificity
Ubiquitous. Expressed in various cancer cell lines. Expressed at very low levels in normal breast epithelial cells and highly expressed in breast tumors. Expression is strongly associated with an aggressive phenotype of breast tumors and expression correlates with tumor size and the metastatic state of the tumor progression. -
Sequence similarities
Contains 1 death domain. -
Domain
An intact death domain is needed for apoptosis. -
Post-translational
modificationsExpression is altered specifically during apoptosis and is accompanied by the appearance of novel forms with smaller apparent molecular mass. -
Cellular localization
Cytoplasm and Nucleus speckle. Nucleus > nucleoplasm. Nucleus matrix. Cytoplasm. Can shuttle between the nucleus and cytoplasm. Nuclear localization is required for induction of apoptotic cell death. Translocates to the cytoplasm during the early phase of apoptosis execution. - Information by UniProt
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Database links
- Entrez Gene: 9984 Human
- Omim: 606930 Human
- SwissProt: Q96FV9 Human
- Unigene: 712543 Human
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Form
Nuclear (Isoform 1) and Cytoplasmic (Isoform 1 and 2). -
Alternative names
- hTREX84 antibody
- Death domain containing protein p84N5 antibody
- HPR 1 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nuclear Matrix Protein p84 antibody (ab245671)
Formalin-fixed, paraffin-embedded human ovarian carcinoma tissue stained for Nuclear Matrix Protein p84 with ab245671 at a 1/200 dilution in immunohistochemical analysis.
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Western blot - Anti-Nuclear Matrix Protein p84 antibody (ab245671)All lanes : Anti-Nuclear Matrix Protein p84 antibody (ab245671) at 0.04 µg/ml
Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
Lysates/proteins at 15 µg per lane.
Predicted band size: 76 kDa
Exposure time: 3 minutesPrepared using NETN lysis buffer.
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Immunoprecipitation - Anti-Nuclear Matrix Protein p84 antibody (ab245671)Nuclear Matrix Protein p84 was immunoprecipitated from HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg per IP reaction; 20% of IP loaded) prepared using NETN lysis buffer.
ab245671 used for IP at 3 µg per reaction. For WB 0.4 µg/ml.
Lane 1: ab245671 IP in HeLa whole cell lysate.
Lane 2: Control IgG in HeLa whole cell lysate.Chemiluminescence detection: 3 seconds.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
References (0)
ab245671 has not yet been referenced specifically in any publications.
