Overview

  • Product name
    Anti-Nuclear Pore Complex Proteins antibody [414]
    See all Nuclear Pore Complex Proteins primary antibodies
  • Description
    Mouse monoclonal [414] to Nuclear Pore Complex Proteins
  • Host species
    Mouse
  • Tested applications
    Suitable for: WB, IP, Electron Microscopy, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human, Saccharomyces cerevisiae, Xenopus laevis
  • Immunogen

    rat liver nuclei extract

  • Positive control
    • HeLa total cell extract.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.40
    Preservative: 0.097% Sodium azide
    Constituent: 0.0268% PBS
  • Concentration information loading...
  • Clonality
    Monoclonal
  • Clone number
    414
  • Myeloma
    NS1
  • Isotype
    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab50008 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 106 kDa.
IP Use at an assay dependent dilution.
Electron Microscopy Use at an assay dependent dilution.
ICC/IF Use at an assay dependent dilution.

Target

Images

  • Lane 1 : Anti-Nuclear Pore Complex Proteins antibody [414] (ab50008) at 1 µg/ml
    Lane 2 : Negative control - Goat Anti-Mouse IgG only

    Lane 1 : Extract from HeLa cells


    Secondary
    Lane 1 : Goat Anti-Mouse IgG


    Predicted band size: 106 kDa
    Observed band size: 150 kDa
    why is the actual band size different from the predicted?

  • ab50008 (1:500, 1hr/ 37oC) staining Nuclear pore complex proteins in HeLa cells (green). Cells were fixed in PHEM buffer (45 mM Hepes pH 6.9, 45 mM Pipes pH 6.9, 5 mM MgCl2, 10 mM EGTA, 4% PFA) /0,2% Triton x-100, 10 min at room T°, then quickly wash 3 x in PBS. ) Good results are also obtained after straight PFA fix: fix in 3.7% PFA / 30 mM sucrose for 10 min at room T°, quickly wash 3 x in PBS, then incubatein PBS/ 0.1% TritonX-100 for 5 min and further in 0.1 M glycine for 10 min. Image kindly provided by R. Mangiacasale,M. Ciciarello and P. Lavia, Institute of. Molecular Biology and Pathology, CNR - Rome (Italy)

References

This product has been referenced in:
  • Takaki T  et al. Actomyosin drives cancer cell nuclear dysmorphia and threatens genome stability. Nat Commun 8:16013 (2017). Read more (PubMed: 28737169) »
  • Eisch V  et al. Progerin impairs chromosome maintenance by depleting CENP-F from metaphase kinetochores in Hutchinson-Gilford progeria fibroblasts. Oncotarget 7:24700-18 (2016). WB, ICC/IF ; Human . Read more (PubMed: 27015553) »
See all 11 Publications for this product

Customer reviews and Q&As

1-4 of 4 Abreviews or Q&A

Answer

I am very pleased to hear you would like to accept our offer and test one or both of these products in blocking experiements. Each code is associated with a paricular antibody so be sure tho reference the correct code when ordering and submitting the Abreview. Each will give you: 1 free PRIMARY ANTIBODY before the expiration date. To redeem this offer, please submit an Abreview and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews. Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code. Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research. The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

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Answer

Thank you very much for your interest in our Nuclear Pore Complex Proteins antibodies. I have spoken to the labs about these product and have found that these are indeed the same clone detailed inin the papers by Davis & Blobel as we spoke of.

We do not currently have a testing data using our Nuclear Pore Complex Proteins antibodies, ab24609 or ab50008in [blocking experiments. Therefore, I can offer a discount off a future purchase if you buy one of these products now, use it and submit an image of your results to us in the form of an Abreview. The discount would be to the value of 1 free PRIMARY ANTIBODY.

It doesn’t matter whether the result is positive or negative, we would just really like to receive your feedback. Of course, if the results are negative you will be covered by our Abpromise guarantee and eligible for a refund or replacement.

If you are interested in this offer, please follow these steps:

1. Reply to this e-mail to let me know that you would like to proceed to test and submit an Abreview in a blocking experiment. I will then send a discount code. This code must be issued before purchasing so please wait for my reply before ordering.

2. Purchase either by phone, fax, or online (www.abcam.com).

3. Use it in your blocking experiment.

4. Submit an Abreview of the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.

5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any PRIMARY ANTIBODY ordered and the discount code is valid for 4 months after issue.
Please remember that submission of the Abreview is sufficient for the discount code to become active.

We are always pleased to obtain feedback about our products and any information is greatly appreciated!

Please let me know which product you would like to test and if you have any questions about this offer and I would be happy to help you further.

The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

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Answer

Thank you for your enquiry. I can confirm this antibody is sourced externally. The originator has confirmed that it will recognise several proteins in the nuclear pore complex in various species. These proteins range in size between 62 and 210 kDa. Unfortunately they do not have any information to indicate the proteins that each band corresponds to. They have performed blotting with HeLa cell extract and find banding between 60 and 150kDa. I am sorry there is regrettably no more information to help you with your enquiry on this occasion. If you have any further questions, please do not hesitate to contact us.

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Application
Immunocytochemistry/ Immunofluorescence
Sample
Kangaroo Cell (rat kangaroo kidney epithelial cell line PtK2)
Specification
rat kangaroo kidney epithelial cell line PtK2
Fixative
Paraformaldehyde
Permeabilization
Yes - Triton X-100
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 20°C

Siegfried Reipert

Verified customer

Submitted Sep 09 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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