Product nameAnti-Nuclear Pore Complex Proteins antibody 
See all Nuclear Pore Complex Proteins primary antibodies
DescriptionMouse monoclonal  to Nuclear Pore Complex Proteins
Tested applicationsSuitable for: WB, IP, Electron Microscopy, ICC/IFmore details
Species reactivityReacts with: Mouse, Rat, Human, Saccharomyces cerevisiae, Xenopus laevis
rat liver nuclei extract
- HeLa total cell extract.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.097% Sodium azide
Constituent: 0.0268% PBS
Concentration information loading...
Our Abpromise guarantee covers the use of ab50008 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 106 kDa.|
|IP||Use at an assay dependent dilution.|
|Electron Microscopy||Use at an assay dependent dilution.|
|ICC/IF||Use at an assay dependent dilution.|
FunctionEssential component of nuclear pore complex. Required for the assembly of peripheral proteins into the nuclear pore complex.
Sequence similaritiesBelongs to the nucleoporin Nup84/Nup107 family.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localizationNucleus > nuclear pore complex. Chromosome > centromere > kinetochore. Located on both the cytoplasmic and nuclear sides of the nuclear pore. During mitosis, localizes to the kinetochores.
- Information by UniProt
- 107 kDa nucleoporin antibody
- NPC proteins antibody
- NU107_HUMAN antibody
Lane 1 : Anti-Nuclear Pore Complex Proteins antibody  (ab50008) at 1 µg/ml
Lane 2 : Negative control - Goat Anti-Mouse IgG only
Lane 1 : Extract from HeLa cells
Lane 1 : Goat Anti-Mouse IgG
Predicted band size: 106 kDa
Observed band size: 150 kDa why is the actual band size different from the predicted?
ab50008 (1:500, 1hr/ 37oC) staining Nuclear pore complex proteins in HeLa cells (green). Cells were fixed in PHEM buffer (45 mM Hepes pH 6.9, 45 mM Pipes pH 6.9, 5 mM MgCl2, 10 mM EGTA, 4% PFA) /0,2% Triton x-100, 10 min at room T°, then quickly wash 3 x in PBS. ) Good results are also obtained after straight PFA fix: fix in 3.7% PFA / 30 mM sucrose for 10 min at room T°, quickly wash 3 x in PBS, then incubatein PBS/ 0.1% TritonX-100 for 5 min and further in 0.1 M glycine for 10 min. Image kindly provided by R. Mangiacasale,M. Ciciarello and P. Lavia, Institute of. Molecular Biology and Pathology, CNR - Rome (Italy)
This product has been referenced in:
- Takaki T et al. Actomyosin drives cancer cell nuclear dysmorphia and threatens genome stability. Nat Commun 8:16013 (2017). Read more (PubMed: 28737169) »
- Eisch V et al. Progerin impairs chromosome maintenance by depleting CENP-F from metaphase kinetochores in Hutchinson-Gilford progeria fibroblasts. Oncotarget 7:24700-18 (2016). WB, ICC/IF ; Human . Read more (PubMed: 27015553) »