Anti-Nuclear Pore Complex Proteins antibody [Mab414] - ChIP Grade (ab24609)

Mouse monoclonal Nuclear Pore Complex Proteins antibody [Mab414]. Validated in WB, IP, IHC, ChIP, EM, ICC/IF and tested in Mouse, Rat, Cat, Human, Saccharomyces cerevisiae, Xenopus laevis and more.

Overview

  • Product name
    Anti-Nuclear Pore Complex Proteins antibody [Mab414] - ChIP Grade
    See all Nuclear Pore Complex Proteins primary antibodies
  • Description
    Mouse monoclonal [Mab414] to Nuclear Pore Complex Proteins - ChIP Grade
  • Host species
    Mouse
  • Tested applications
    Suitable for: ChIP, WB, ICC/IF, IP, Electron Microscopy, IHC-Frmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Cat, Human, Saccharomyces cerevisiae, Xenopus laevis, Caenorhabditis elegans, Drosophila melanogaster, Zebrafish
    Predicted to work with: Vertebrata
  • Immunogen

    Nuclear pore complex mixture.

  • Epitope
    ab24609 recognizes the conserved domain FXFG repeats in nucleaporins like the p62, p152, p90.
  • Positive control
    • Raw, HEK 293 cell lysate (see Abreview), rat liver lysate (see Aris reference)
  • General notes

     

    This is a reliable general purpose monoclonal antibody which recognizes a related family of NPC proteins. This antibody is ideal for studying the morphology and composition of the nucleus and nuclear envelope. It is also useful in studying changes in the nuclear structure during mitosis and meiosis.

    This product was changed from ascites to tissue culture supernatant on 20/05/2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.03% Thimerosal (merthiolate)
    Constituent: PBS
  • Purity
    Tissue culture supernatant
  • Primary antibody notes
    This is a reliable general purpose monoclonal antibody which recognizes a related family of NPC proteins. This antibody is ideal for studying the morphology and composition of the nucleus and nuclear envelope. It is also useful in studying changes in the nuclear structure during mitosis and meiosis.
  • Clonality
    Monoclonal
  • Clone number
    Mab414
  • Isotype
    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab24609 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ChIP Use at an assay dependent concentration. PubMed: 20419146
WB Use at an assay dependent concentration. Predicted molecular weight: 62 kDa.

Suitable also in non reduced western blotting conditions (see Abreview). Nuclear extraction may be necessary (protocol detailed in Aris et al, http://www.jcb.org/cgi/reprint/108/6/2059).

ICC/IF Use at an assay dependent concentration.

Fix cells with 4% paraformaldehyde in NWB (200 mM sucrose, 15 mM Hepes, pH 7.4, 50 mM NaCl, 2.5 mM MgCl2, and 1 mM DTT). Permeabilise with 0.1% NP-40 or 0.1% Triton X-100 in PBS for 2 min. (see Lopez-Soler reference); different customer have used this antibody at different dilutions for ICC/IF (see images below). We recommend that optimal working dilutions are determined by each customer.

IP Use at an assay dependent concentration.

Nuclear extraction may be necessary (protocol detailed in Aris et al, http://www.jcb.org/cgi/reprint/108/6/2059).

Electron Microscopy Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration. 4% paraformaldehyde fixed tissue cut on a cryostat (see Kimura reference).

Target

Images

  • ab24609 staining nuclear pore complex proteins in Human chronic myelogenous leukemia cells from bone marrow cells. Cells were fixed with 4% paraformaldehyde in DPBS buffer for 20 mins and permeabilised with 0.1% Triton X-100 for 20 min at room temperature. Cells were blocked with 2% normal goat serum in DPBS with 1% BSA and washed with 0.1% Tween 20.

    This image was generated using the ascites version of the product.

  • ab24609 (1/200) staininig nuclear pore complex proteins in human RPE-1 cells (green). Cells were fixed in paraformaldehyde, permeabilised with Triton X100 and counterstained with DAPI in order to highlight the nucleus (blue). Please refer to abreview for further experimental details.

    This image was generated using the ascites version of the product.

    See Abreview

  • ab24609 (1/500) staining nuclear pore complex proteins in human Hela Cells (green). Cells were fixed with Paraformaldehyde/Triton X-100 (10 min in PTEMF buffer (20mM PIPES, 1mM MgCl2, 10mM EGTA, 4% PFA) /0.2% Triton-X100 at room T°C) or Methanol (6 min in Methanol -20 °C , followed by 3 washes in 1x PBS) and counterstained with Dapi in order to highligh the nucleus (blue).

    This image was generated using the ascites version of the product.

  • ab24609 (1/500) staining nuclear pore complex proteins in murine NIH/3T3 Cells (green). Cells were fixed with Paraformaldehyde/Triton X-100 (10 min in PTEMF buffer (20mM PIPES, 1mM MgCl2, 10mM EGTA, 4% PFA) /0.2% Triton-X100 at room T°C) or Methanol (6 min in Methanol -20 °C , followed by 3 washes in 1x PBS) and counterstained with Dapi in order to highligh the nucleus (blue).

    This image was generated using the ascites version of the product.

References

This product has been referenced in:
  • Bejarano DA  et al. HIV-1 nuclear import in macrophages is regulated by CPSF6-capsid interactions at the nuclear pore complex. Elife 8:N/A (2019). Read more (PubMed: 30672737) »
  • Pereira C  et al. Self-Assembly of the RZZ Complex into Filaments Drives Kinetochore Expansion in the Absence of Microtubule Attachment. Curr Biol 28:3408-3421.e8 (2018). Read more (PubMed: 30415699) »
See all 74 Publications for this product

Customer reviews and Q&As

21-21 of 21 Abreviews or Q&A

Application
Western blot
Sample
Human Cell lysate - other (Raw, HEK 293,)
Loading amount
100000 cells
Specification
Raw, HEK 293,
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10%

Dr. Alain Chariot

Verified customer

Submitted Jul 27 2006

21-21 of 21 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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