Question (70881) | Anti-Nuclear Pore Complex Proteins antibody [Mab414] - ChIP Grade (ab24609)

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Question

ICC/IF with human epithelial cells
sees punctuated pattern in the nulceus, not the expected nuclear membrane staining
Fix: 4% PFA or 3.7% FA
Ab: 1/1000 - 1/20,000
block: 2% BSA or BSA/serum
perm: TX100
2nd: works well
tried different protocols

Answer

issued a free of charge replacement

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