Immunocytochemistry/ Immunofluorescence abreview for Anti-Nuclear Pore Complex Proteins antibody [Mab414] - ChIP Grade

Good
Abreviews
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (U2Os)
Specification
U2Os
Fixative
Formaldehyde
Permeabilization
Yes - 0.1% Triton in PBS
Blocking step
FCS in PBS as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 22°C

Other product details

Dilution
1/1000
Incubation time
45 minute(s) · Temperature: 22°C · Diluent: 1x PBS

Secondary antibody

Name
Non-Abcam antibody was used: Alexa Fluor® 647 F(ab')2 Fragment of Goat Anti-Mou
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 647
Dilution
1/200

Additional data

Additional Notes
This is a super-resolution image of an U2Os nucleus labeled with mAb414 antibodies and Alexa 647 conjugated F(ab')2 fragments. We used direct stochastic optical reconstruction microscopy (dSTORM) to resolve the single nuclear pores.

Ms. Anna Löschberger

Verified customer

Submitted Oct 15 2012

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