Western blot

Excellent
Abreviews
Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing
Sample
Human Cell lysate - nuclear (U2OS)
Specification
U2OS
Blocking step
Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C

Other product details

Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% milk in TBST
Dilution
1/4000

Secondary antibody

Name
Non-Abcam antibody was used: anti-mouse
Host species: Goat
Clonality: Polyclonal
Conjugation: IRDye® 680
Dilution
1/10000

Detection

Detection method
Fluorescence detection
Bands
Specific: 62, 110 kDa
Exposure
5 minute(s) and 0 second(s)

Abcam user community

Verified customer

Submitted Jun 04 2013

Sign up