Western blot abreview for Anti-Nuclear Pore Complex Proteins antibody [Mab414] - ChIP Grade

Good
Abreviews
Application
Western blot
Sample
Human Cell lysate - other (Raw, HEK 293,)
Loading amount
100000 cells
Specification
Raw, HEK 293,
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10%

Other product details

Dilution
1/5000
Incubation time
2 hour(s) and 0 minute(s)

Secondary antibody

Name
Non-Abcam antibody was used: ECL TM anti-mouse IgG
Host species: Sheep
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
ECL+
Exposure
15 minute(s) and 0 second(s)
Bands
Specific: 62 kDa Non-specific: 170, 130, 43, 26 kDa

Dr. Alain Chariot

Verified customer

Submitted Jul 27 2006

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