Perfect antibody for Nuclear pore complex staining. Works best for IFA at 1:5000 dilution.
1. Perform CSK digestion before Paraformaldehyde fixation (to digest cytoplasm).
2. 10 min PFA fixation
3. 30 min blocking in 1% BSA
4. 1 hour 30 min incubation at RT in a moist chamber.
5. 1 Wash with 1X PBST followed by 2 washes with 1X PBS at RT.
6. Secondary with Goat Anti-Mouse IgG Alexa 488.
Abcam user community
Submitted Jan 24 2017
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