For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

We use cookies to make our site as useful as possible.

Our Cookie Policy explains how you can opt-out of the cookies we use.

If you continue without changing your cookie settings, we'll assume you’re happy with this.

Continue Continue

United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Diagnostic & Therapeutic Solutions
    Custom solutions & partnerships

    Custom antibody development and commercial partnerships to advance your diagnostic and therapeutic discovery.

    Create custom solutions with us

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

Supporting our customers and employees during the COVID-19 pandemic. Read more

  1. Link

    nuclear-pore-o-linked-glycoprotein-antibody-rl1-ab2734.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Tags & Cell Markers Subcellular Markers Nucleus Nuclear Pore
Share by email

Anti-Nuclear Pore O-Linked Glycoprotein antibody [RL1] (ab2734)

  • Datasheet
  • SDS
Submit a review Q&A (2)References (7)

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nuclear Pore O-Linked Glycoprotein antibody [RL1] (ab2734)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nuclear Pore O-Linked Glycoprotein antibody [RL1] (ab2734)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nuclear Pore O-Linked Glycoprotein antibody [RL1] (ab2734)

Key features and details

  • Mouse monoclonal [RL1] to Nuclear Pore O-Linked Glycoprotein
  • Suitable for: IHC-P
  • Reacts with: Rat, Saccharomyces cerevisiae
  • Isotype: IgM

Overview

  • Product name

    Anti-Nuclear Pore O-Linked Glycoprotein antibody [RL1]
  • Description

    Mouse monoclonal [RL1] to Nuclear Pore O-Linked Glycoprotein
  • Host species

    Mouse
  • Specificity

    Detects nuclear pore-O-linked glycoprotein
  • Tested Applications & Species

    Application Species
    IHC-P
    Rat
    See all applications and species data
  • Immunogen

    Other Immunogen Type corresponding to Rat Nuclear Pore O-Linked Glycoprotein. Pore complex-lamina fraction purified from rat liver nuclear envelopes.

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.05% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Purified IgM
  • Clonality

    Monoclonal
  • Clone number

    RL1
  • Isotype

    IgM
  • Research areas

    • Tags & Cell Markers
    • Subcellular Markers
    • Nucleus
    • Nuclear Pore
    • Signal Transduction
    • Protein Trafficking
    • Nuclear Import / Export

Associated products

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Rabbit Anti-Mouse IgG+IgM+IgA H&L (ab8516)
    • Rabbit Anti-Mouse IgG+IgM+IgA H&L (FITC) (ab8517)
    • Goat Anti-Mouse IgM H&L (ab9167)
    • Rabbit Anti-Mouse IgM mu chain (ab9175)
  • Isotype control

    • Mouse IgM [B11/7] - Isotype control (ab91545)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab2734 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
IHC-P
Rat
All applications
Mammals
Application Abreviews Notes
IHC-P
Use at an assay dependent concentration.
Notes
IHC-P
Use at an assay dependent concentration.

Target

  • Relevance

    Diffusion of metabolites and small non-nuclear molecules as well as active, mediated import of protein and export of protein and RNA through the nuclear envelope occurs through nuclear pore complexes or NPC’s. NPC’s contain up to 100 different polypeptides which have a combined mass of about 125 megadaltons. The channel available for passive transport through the NPC is about 9-10 nm in diameter while carrier mediated changes in the NPC result in a ~25 nm channel used for larger, actively transported molecules. Of the 100 polypeptides, at least 8 of these are O-linked N-acetylglycosamine-modified in mammalian cells. All of the mammalian O-linked glycoproteins contain multiple copies of phenylalanine, glycine dipeptide repeats dispersed throughout part of their sequence. Studies indicate that the NPC O-linked glycoproteins have a direct role in nuclear protein import.
  • Cellular localization

    Nuclear membrane

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nuclear Pore O-Linked Glycoprotein antibody [RL1] (ab2734)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nuclear Pore O-Linked Glycoprotein antibody [RL1] (ab2734)
    Immunohistochemistry was performed on normal biopsies of deparaffinized Rat lymph node tissue. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:200 with a mouse monoclonal antibody recognizing Nuclear Pore-O-Linked Glycoprotein ab2734 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nuclear Pore O-Linked Glycoprotein antibody [RL1] (ab2734)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nuclear Pore O-Linked Glycoprotein antibody [RL1] (ab2734)
    Immunohistochemistry was performed on normal biopsies of deparaffinized Rat brain tissue. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:200 with a mouse monoclonal antibody recognizing Nuclear Pore-O-Linked Glycoprotein ab2734 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nuclear Pore O-Linked Glycoprotein antibody [RL1] (ab2734)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nuclear Pore O-Linked Glycoprotein antibody [RL1] (ab2734)
    Immunohistochemistry was performed on normal biopsies of deparaffinized Rat kidney tissue. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:200 with a mouse monoclonal antibody recognizing Nuclear Pore-O-Linked Glycoprotein ab2734 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

Protocols

  • Immunohistochemistry protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (7)

Publishing research using ab2734? Please let us know so that we can cite the reference in this datasheet.

ab2734 has been referenced in 7 publications.

  • Gorsch LC  et al. A conditional allele of the novel repeat-containing yeast nucleoporin RAT7/NUP159 causes both rapid cessation of mRNA export and reversible clustering of nuclear pore complexes. J Cell Biol 129:939-55 (1995). PubMed: 7744966
  • Guan T  et al. Structural analysis of the p62 complex, an assembly of O-linked glycoproteins that localizes near the central gated channel of the nuclear pore complex. Mol Biol Cell 6:1591-603 (1995). PubMed: 8589458
  • Byrd DA  et al. Tpr, a large coiled coil protein whose amino terminus is involved in activation of oncogenic kinases, is localized to the cytoplasmic surface of the nuclear pore complex. J Cell Biol 127:1515-26 (1994). PubMed: 7798308
  • Greber UF & Gerace L Nuclear protein import is inhibited by an antibody to a lumenal epitope of a nuclear pore complex glycoprotein. J Cell Biol 116:15-30 (1992). PubMed: 1370490
  • Sterne-Marr R  et al. O-linked glycoproteins of the nuclear pore complex interact with a cytosolic factor required for nuclear protein import. J Cell Biol 116:271-80 (1992). PubMed: 1730755
  • Holt GD  et al. Nuclear pore complex glycoproteins contain cytoplasmically disposed O-linked N-acetylglucosamine. J Cell Biol 104:1157-64 (1987). PubMed: 3571327
  • Snow CM  et al. Monoclonal antibodies identify a group of nuclear pore complex glycoproteins. J Cell Biol 104:1143-56 (1987). PubMed: 2437126

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

1-2 of 2 Abreviews or Q&A

Question

I was just wondering if you know what nuclear pore proteins this antibody is detecting in yeast.

Read More

Abcam community

Verified customer

Asked on Apr 13 2007

Answer

Thank you for your enquiry. I can confirm that this product detects nuclear pore-O-linked glycoprotein from rat, amphibian and yeast. I hope this information will be useful to you. If you require any further information, please do not hesitate to contact me again.

Read More

Abcam Scientific Support

Answered on Apr 13 2007

Question

Which NPC proteins does this antibody detect? I am also seeing a 250kDa band on my Western blot. Has this band been observed before?

Read More

Abcam community

Verified customer

Asked on Mar 06 2006

Answer

Thank you for your enquiry. I am unaware of any resources that mention a nuclear pore protein at 250 kDa. I did a quick literature search and did not pull up a nuclear pore protein at 250 kDa. However, I did find an interesting article that states a 250 kDa band may be another glycoprotein that is immunoprecipitated along with Nuclear pore protein 68 (pBC68). I have added the link below to where you can get a free full-text article. http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_ui ds=10462521&dopt=Abstract Please do not hesitate to contact us if you need anything further.

Read More

Abcam Scientific Support

Answered on Mar 07 2006

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Get resources and offers direct to your inbox Sign up
A-Z by research area
  • Cancer
  • Cardiovascular
  • Cell biology
  • Developmental biology
  • Epigenetics & Nuclear signaling
  • Immunology
  • Metabolism
  • Microbiology
  • Neuroscience
  • Signal transduction
  • Stem cells
A-Z by product type
  • Primary antibodies
  • Secondary antibodies
  • Biochemicals
  • Isotype controls
  • Flow cytometry multi-color selector
  • Kits
  • Loading controls
  • Lysates
  • Peptides
  • Proteins
  • Slides
  • Tags and cell markers
  • Tools & Reagents
Help & support
  • Support
  • Make an Inquiry
  • Protocols & troubleshooting
  • Placing an order
  • RabMAb products
  • Biochemical product FAQs
  • Training
  • Browse by Target
Company
  • Corporate site
  • Investor relations
  • Company news
  • Careers
  • About us
  • Blog
Events
  • Tradeshows
  • Conferences
International websites
  • abcam.cn
  • abcam.co.jp

Join with us

  • LinkedIn
  • facebook
  • Twitter
  • YouTube
  • Terms of sale
  • Website terms of use
  • Cookie policy
  • Privacy policy
  • Legal
  • Modern slavery statement
© 1998-2021 Abcam plc. All rights reserved.