For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

We use cookies to make our site as useful as possible.

Our Cookie Policy explains how you can opt-out of the cookies we use.

If you continue without changing your cookie settings, we'll assume you’re happy with this.

Continue Continue

United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Diagnostic & Therapeutic Solutions
    Custom solutions & partnerships

    Custom antibody development and commercial partnerships to advance your diagnostic and therapeutic discovery.

    Create custom solutions with us

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

Supporting our customers and employees during the COVID-19 pandemic. Read more

  1. Link

    nuclear-receptor-corepressor-ncor-antibody-ab58396.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Epigenetics and Nuclear Signaling Chromatin Modifying Enzymes Acetylation
Share by email

Anti-Nuclear Receptor Corepressor NCoR antibody (ab58396)

  • Datasheet
Submit a review Q&A (6)References (3)

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Western blot - Anti-Nuclear Receptor Corepressor NCoR antibody (ab58396)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nuclear Receptor Corepressor NCoR antibody (ab58396)

Key features and details

  • Rabbit polyclonal to Nuclear Receptor Corepressor NCoR
  • Suitable for: WB, IHC-P
  • Reacts with: Human
  • Isotype: IgG

You may also be interested in

Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
Protein
Recombinant human Nuclear Receptor Corepressor NCoR protein (ab82239)

View more associated products

Overview

  • Product name

    Anti-Nuclear Receptor Corepressor NCoR antibody
    See all Nuclear Receptor Corepressor NCoR primary antibodies
  • Description

    Rabbit polyclonal to Nuclear Receptor Corepressor NCoR
  • Host species

    Rabbit
  • Specificity

    This antibody detects endogenous levels of total NCoR1 protein.
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide derived from the internal sequence of Nuclear Receptor Corepressor NCoR (Human)

  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride

    Without Mg+2 and Ca+2
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    Purified from rabbit antiserum.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Chromatin Modifying Enzymes
    • Acetylation
    • Signal Transduction
    • Signaling Pathway
    • Nuclear Signaling
    • Nuclear Hormone Receptors
    • Co-activators/co-repressors
    • Epigenetics and Nuclear Signaling
    • Nuclear Signaling Pathways
    • Nuclear Receptors
    • Co-activators/co-repressors
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Co-factors
    • Epigenetics and Nuclear Signaling
    • Chromatin Modifying Enzymes
    • Acetylation
    • HDACs
    • Class II / Hda1 Class
    • Cancer
    • Signal transduction
    • Nuclear signaling
    • Nuclear hormone receptors
    • Coactivators and repressors
    • Cancer
    • Cancer Metabolism
    • Metabolic signaling pathway
    • Metabolism of lipids and lipoproteins
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Lipid and lipoprotein metabolism
    • Lipid metabolism
    • Metabolism
    • Pathways and Processes
    • Cofactors, Vitamins / minerals
    • Co-factors
    • Metabolism
    • Types of disease
    • Cancer

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant human Nuclear Receptor Corepressor NCoR protein (ab82239)

Applications

Our Abpromise guarantee covers the use of ab58396 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 270 kDa.
IHC-P 1/50 - 1/100.

Target

  • Function

    Mediates transcriptional repression by certain nuclear receptors. Part of a complex which promotes histone deacetylation and the formation of repressive chromatin structures which may impede the access of basal transcription factors.
  • Sequence similarities

    Belongs to the N-CoR nuclear receptor corepressors family.
    Contains 2 SANT domains.
  • Domain

    The N-terminal region contains three independent domains that are capable of mediating transcriptional repression (RD1, RD2 and RD3).
    The C-terminal region contains two separate nuclear receptor-interacting domains (ID1 and ID2), each of which contains a conserved sequence referred to as the CORNR box. This motif is necessary and sufficient for binding to unligated nuclear hormone receptors, while sequences flanking the CORNR box determine the precise nuclear hormone receptor specificity.
  • Post-translational
    modifications

    Ubiquitinated; mediated by SIAH2 and leading to its subsequent proteasomal degradation.
  • Cellular localization

    Nucleus.
  • Target information above from: UniProt accession O75376 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 9611 Human
    • Omim: 600849 Human
    • SwissProt: O75376 Human
    • Unigene: 462323 Human
    • Alternative names

      • hN CoR antibody
      • hNCoR antibody
      • KIAA1047 antibody
      • N CoR antibody
      • N Cor/SMRT corepressor Rip13 antibody
      • N CoR1 antibody
      • N-CoR antibody
      • N-CoR1 antibody
      • NCOR 1 antibody
      • NCoR antibody
      • Ncor1 antibody
      • NCOR1_HUMAN antibody
      • Nuclear receptor co repressor 1 antibody
      • Nuclear receptor corepressor 1 antibody
      • Retinoid X receptor interacting protein 13 antibody
      • RIP13 antibody
      • Rxrip13 antibody
      • thyroid hormone and retinoic acid receptor associated corepressor 1 antibody
      • TRAC 1 antibody
      • TRAC1 antibody
      see all

    Images

    • Western blot - Anti-Nuclear Receptor Corepressor NCoR antibody (ab58396)
      Western blot - Anti-Nuclear Receptor Corepressor NCoR antibody (ab58396)
      All lanes : Anti-Nuclear Receptor Corepressor NCoR antibody (ab58396) at 1/500 dilution

      Lane 1 : MDA-MB-435 cell extract with no immunizing peptide
      Lane 2 : MDA-MB-435 cell extract with immunizing peptide

      Predicted band size: 270 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nuclear Receptor Corepressor NCoR antibody (ab58396)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nuclear Receptor Corepressor NCoR antibody (ab58396)
      ab58396, at 1/50 diltuion, staining human Nuclear Receptor Corepressor NCoR in breast carcinoma tissue by immunohistochemistry in the presence or absence of the immunizing peptide. Paraffin embedded tissue.

    Protocols

    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (3)

    Publishing research using ab58396? Please let us know so that we can cite the reference in this datasheet.

    ab58396 has been referenced in 3 publications.

    • Figueroa J  et al. Exosomes from Glioma-Associated Mesenchymal Stem Cells Increase the Tumorigenicity of Glioma Stem-like Cells via Transfer of miR-1587. Cancer Res 77:5808-5819 (2017). PubMed: 28855213
    • Gallardo F  et al. Cytoplasmic accumulation of NCoR in malignant melanoma: consequences of altered gene repression and prognostic significance. Oncotarget 6:9284-94 (2015). WB ; Human . PubMed: 25823659
    • Scaffidi P & Misteli T Lamin A-dependent misregulation of adult stem cells associated with accelerated ageing. Nat Cell Biol 10:452-9 (2008). ICC/IF ; Human . PubMed: 18311132

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-6 of 6 Abreviews or Q&A

    Question

    Hi again

    You wanted me to sent you evaluation of your antibodys...but still I have the same problem...non of your antibodys (ab 24552 or ab 58396) detect NCoR1 at the predicted size (270kDa). You can se a band around 250kDa...Can you do some sequencing to really confirm that your antibody picks up the right protein????? I think that the ChIp graded antibody works better and I think that you owe me one new so I can try since I payed for the ab 58396 that does not work as well...

    Are you shore that the protein that your antibodys picks up is not just the isoform 2???

    se pictures from human sk-muscle

    Read More

    Abcam community

    Verified customer

    Asked on Dec 13 2012

    Answer

    Thank you for your reply.

    I am sorry the results you have observed have not improved with ab24552. From looking at the results you have shared you appear to be getting very similar results, with ab24552 showing a much clearer band at ˜250 kDa than with ab58396. As discussed previously, the band at ˜250 kDa may well be the band that you are expecting. Although the SwissProt reference for the Nuclear Receptor Corepressor NCoR states that isoforms of ˜260 and 270 have been observed of this protein. The antibodies you have used have both been raised against regions of the NCoR protein which are present in both isoforms so if either or both of the proteins are expressed in your samples they would be expected to be detected. I have not been able to find any further information as to which of the isoforms is most prevalent. I would therefore not be able offer a suggestion as to which of the two isoforms it may be that you are detecting.

    As the results you are seeing with both antibodies are consistent (both show bands of ˜250 kDa), I would suggests this supports this being a specific band. If you would like to confirm this further you could isolate the protein in the band and perform Mass Spec analysis. Alternatively, if you have access to a construct which can be used to over-express the protein in a cell line this can often prove a useful positive and negative control.

    You are seeing a shift of about 20 kDa in the size of the protein on your membrane. This size of shift is not uncommon for proteins of this size. Separation by SDS-PAGE does not give the exact size of proteins. It relies on the proteins being coated in SDS proportional to their size, providing a negative charge proportional to their size. This is not always the case. The degree of SDS binding the protein can be affected by its hydrophobicity, charge, glycosylation, phosphorylation, or simply the amino acid sequence. This can also be different depending on the lysis buffer and running buffers used when running the gel.

    I hope this information has been of help. If you would like for me to help optimise the results, if you could possibly provide me with more details of how you are running the experiment now. I have attached our questionnaire for this reason. Could you also detail how the tissue is being processed (which lysis buffer and the running buffers used)?

    I look forward to receiving your reply.

    Read More

    Abcam Scientific Support

    Answered on Dec 13 2012

    Question

    1. Would you be able to let me know that the ladder used was and what the two main bands correspond to? Are these ˜150 kDa and ˜250 kDa as you have mentioned before?

    2. What is the difference between the blot on the left hand side and the right hand side?

    3. What samples are you looking at currently (human/mouse, cell/tissue lysate)?

    For your questions
    1) The ladder that is seen are made from BioRad DualColor and that band are as you suspected 150kDa and 250kDa.
    2) The blot is just a testing/optimization blot so the difference on the left and right side is due to different samples etc.



    3) These samples is human sk-muscle, adn we use Li-Core Oddysey scanning system and BioRad Turbo Transblot system for the transfer.


    So my final question is; Do you think that the band Im picking up around 250kDa is the isoform 2 of NCoR1...?




    Eva-karin Sällstedt
    Please consider the environment before printing this email...

    Department of Physiology and Pharmacology
    (Molecular Exercise Physiology)
    Karolinska Institutet
    von Eulers väg 8
    S-171 77 Stockholm


    Cell: +46 70 33 977 32

    Phone: +46 8 524 868 74

    Read More

    Abcam community

    Verified customer

    Asked on Oct 30 2012

    Answer

    Thank you for your reply and I am sorry for the delay in my response.

    In answer to your questions, I think the upper band you are seeing may well be the Nuclear Receptor Corepressor NCoR protein. It seems from your blot that there may be some further optimisation required in the transfer of the protein. With such a large target it is vital to get good transfer of higher molecular weigh proteins. This may be improved by using the following tips:

    1. Make sure to use a low percentage gel (preferably 8% or less).

    2. Large proteins will tend to precipitate in the gel, hindering transfer. Adding SDS to a final concentration of 0.1% in the transfer buffer will discourage this. Methanol tends to remove SDS from proteins, so reducing the methanol percentage to 10% or less will also guard against precipitation. This also promotes swelling of the gel, allowing large proteins to transfer more easily. If using a PVDF membrane I would suggest removing methanol from the transfer buffer altogether (with PVDF methanol is only needed to activate the membrane before assembling the gel/membrane sandwich).

    3. I would suggest trying wet transfer overnight at 4°C instead of semi-dry transfer.

    4. Ponceau red can be used to check for efficient transfer of the proteins.

    From your membrane I would also suggest reducing the quantity of protein loaded on the gel and the antibody concentration.

    I look forward to receiving your reply.

    Read More

    Abcam Scientific Support

    Answered on Oct 30 2012

    Question

    Hej

    Tror inte att det är något i vårt protokoll som bör ändras, fungerar utmärkt för alla de andra faktorerna som jag testar (där av har jag inte fyllt i ert frågeformulär). Så du skulle gissa att bandet vid 250 kDa skulle motsvara Isoform 2 av NCoR1? Skulle du använda detta band (efter att jag optimerat blotarna) för kvantifiering?

    Read More

    Abcam community

    Verified customer

    Asked on Oct 25 2012

    Answer

    I am sorry for the delay in getting back to you.

    I now have confirmation of the protocol used with the Anti-Nuclear Receptor Corepressor NCoR antibody (ab58396). The Western blot presented on the datasheet of ab58396 was performed using 5% milk blocking in TBST (0.05% Tween) for 1 hour at room temperature. The antibody was then incubated with the primary antibody, diluted 1/1000 in 1% milk in 0.05% TBST at 4°C overnight.

    I hope this information has been of help. If you would like me to take a further look at your results could you please provide me with the following information:

    1. Would you be able to let me know that the ladder used was and what the two main bands correspond to? Are these ˜150 kDa and ˜250 kDa as you have mentioned before?

    2. What is the difference between the blot on the left hand side and the right hand side?

    3. What samples are you looking at currently (human/mouse, cell/tissue lysate)?

    I look forward to receiving your reply.

    Read More

    Abcam Scientific Support

    Answered on Oct 25 2012

    Question

    Hej

    Tror inte att det är något i vårt protokoll som bör ändras, fungerar utmärkt för alla de andra faktorerna som jag testar (där av har jag inte fyllt i ert frågeformulär). Så du skulle gissa att bandet vid 250 kDa skulle motsvara Isoform 2 av NCoR1? Skulle du använda detta band (efter att jag optimerat blotarna) för kvantifiering?

    Read More

    Abcam community

    Verified customer

    Asked on Oct 24 2012

    Answer

    Thank you for your reply.

    Our experience is that some antibodies work much better under certain conditions. For example, the anti-GAPDH antibody ab9385 has been shown to work much more efficiently with BSA used in blocking than milk. This can be viewed from the following link:

    https://www.abcam.com/GAPDH-antibody-HRP-Loading-Control-ab9385.html

    This is why I suggested that I have a look at the protocol used to see if there were any suggestions I could make in order to reduce the background currently observed. I will contact the lab to see if they have any specific suggestions of how the antibody performs at its best.

    It is difficult to make a conclusion about your results so far as I do not know exactly which bands are which on your blot. Would you be able to let me know that the ladder used was and what the two main bands correspond to? Are these ˜150 kDa and ˜250 kDa as you have mentioned before?

    What is the difference between the blot on the left hand side and the right hand side?

    What samples are you looking at currently?

    I will get back to you as soon as I have any additional feedback from the lab.

    Read More

    Abcam Scientific Support

    Answered on Oct 24 2012

    Question

    Hi

    Have not got any response...??? Have you tested your antibody (ab58396) https://www.abcam.com/Nuclear-Receptor-Corepressor-NCoR-antibody-ab58396.html???? are you sure the size is 270kDa??? If so, send me some proof.

    The pictures you show on the website is a drawing more then a blot picture??? I need answers this week so I know how I must continue....

    Read More

    Abcam community

    Verified customer

    Asked on Oct 22 2012

    Answer

    Thank you for your reply.

    I am sorry for the delay in my response. I will reply in English if you don't mind as I am more comfortable discussing scientific content in this language. By all means feel free to reply in Swedish, I will understand.

    The Nuclear Receptor Corepressor NCoR protein is referred to as having a predicted molecular weight of 270 for isoform 1 and 258 kDa for isoform 2 (SwissProt reference O75376). As the protein is also known to undergo a number of different post-translational modifications, this size could vary slightly when viewed on a blot.

    Let me reassure you, the image presented on the datasheet of ab58396 is not a faked image, it is a Western blot run with MDA-MB-435 cell extract (human breast carcinoma). It is difficult to assess the exact size, (whether it is 250 kDa as you have observed, or 270 kDa as is predicted) as the ladder used to run with the sample does not allow accurate quantification.

    Thank you for sharing the results you have obtained with this antibody. It may well be that the band at ˜250 kDa is the Nuclear Receptor Corepressor NCoR and the band at ˜150 kDa is a non-specific binding or the protein (or a degradation product). In order to be able to advise further, I would like to collect a little further information from you in regards to the protocol used and the samples tested.

    To this end, would you mind filling in the questionnaire I have attached to this email? Once I have received the completed questionnaire, I will look at the protocol and see if there are any suggestions I can make that may improve the results. In addition to this, could you label the blot you shared with the molecular weight markers?

    This information will also allow us to investigate this case internally and initiate additional testing where necessary.

    I look forward to receiving your reply.

    Read More

    Abcam Scientific Support

    Answered on Oct 22 2012

    Question

    Could you clarify whether the test were on human or rat samples? Our samples were rat proteins.

    Read More

    Abcam community

    Verified customer

    Asked on Sep 06 2011

    Answer

    The western blot image on the datasheet for ab58396 shows that this antibody was tested on human MDA-MB-435 cells. We have not tested this antibody against rat samples in house, however, based on sequence homology this antibody can pick up a band between 50 and 60 kDa in rat in addition to a band at 270 kDa.

    Read More

    Abcam Scientific Support

    Answered on Sep 06 2011

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Get resources and offers direct to your inbox Sign up
    A-Z by research area
    • Cancer
    • Cardiovascular
    • Cell biology
    • Developmental biology
    • Epigenetics & Nuclear signaling
    • Immunology
    • Metabolism
    • Microbiology
    • Neuroscience
    • Signal transduction
    • Stem cells
    A-Z by product type
    • Primary antibodies
    • Secondary antibodies
    • Biochemicals
    • Isotype controls
    • Flow cytometry multi-color selector
    • Kits
    • Loading controls
    • Lysates
    • Peptides
    • Proteins
    • Slides
    • Tags and cell markers
    • Tools & Reagents
    Help & support
    • Support
    • Make an Inquiry
    • Protocols & troubleshooting
    • Placing an order
    • RabMAb products
    • Biochemical product FAQs
    • Training
    • Browse by Target
    Company
    • Corporate site
    • Investor relations
    • Company news
    • Careers
    • About us
    • Blog
    Events
    • Tradeshows
    • Conferences
    International websites
    • abcam.cn
    • abcam.co.jp

    Join with us

    • LinkedIn
    • facebook
    • Twitter
    • YouTube
    • Terms of sale
    • Website terms of use
    • Cookie policy
    • Privacy policy
    • Legal
    • Modern slavery statement
    © 1998-2021 Abcam plc. All rights reserved.