Anti-Nuclear Receptor Corepressor NCoR antibody (ab3482)
Key features and details
- Rabbit polyclonal to Nuclear Receptor Corepressor NCoR
- Suitable for: ChIP, WB, IP, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Nuclear Receptor Corepressor NCoR antibody
See all Nuclear Receptor Corepressor NCoR primary antibodies -
Description
Rabbit polyclonal to Nuclear Receptor Corepressor NCoR -
Host species
Rabbit -
Tested applications
Suitable for: ChIP, WB, IP, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Xenopus laevis, Xenopus tropicalis -
Immunogen
Synthetic peptide corresponding to Mouse Nuclear Receptor Corepressor NCoR aa 2427-2443.
Sequence:PAPLLSAQYETLSDSDD
(Peptide available asab4997) -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.1% BSA -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
- Signal Transduction
- Signaling Pathway
- Nuclear Signaling
- Nuclear Hormone Receptors
- Co-activators/co-repressors
- Epigenetics and Nuclear Signaling
- Nuclear Signaling Pathways
- Nuclear Receptors
- Co-activators/co-repressors
- Epigenetics and Nuclear Signaling
- Chromatin Modifying Enzymes
- Acetylation
- HDACs
- Class II / Hda1 Class
Associated products
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ChIP Related Products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab3482 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ChIP |
Use at an assay dependent concentration.
Pubmed ID: 16930818. |
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WB | (1) |
Use at an assay dependent concentration. Predicted molecular weight: 270 kDa.
By Western blot, this antibody detects a protein at ~270 kDa representing N-CoR from HeLa cell extracts and mouse RAW 264.7/primary macrophages. |
IP |
Use at an assay dependent concentration.
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IHC-P | (1) |
1/200 - 1/1000.
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ICC/IF |
1/100 - 1/500.
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Notes |
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ChIP
Use at an assay dependent concentration. Pubmed ID: 16930818. |
WB
Use at an assay dependent concentration. Predicted molecular weight: 270 kDa. By Western blot, this antibody detects a protein at ~270 kDa representing N-CoR from HeLa cell extracts and mouse RAW 264.7/primary macrophages. |
IP
Use at an assay dependent concentration. |
IHC-P
1/200 - 1/1000. |
ICC/IF
1/100 - 1/500. |
Target
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Function
Mediates transcriptional repression by certain nuclear receptors. Part of a complex which promotes histone deacetylation and the formation of repressive chromatin structures which may impede the access of basal transcription factors. -
Sequence similarities
Belongs to the N-CoR nuclear receptor corepressors family.
Contains 2 SANT domains. -
Domain
The N-terminal region contains three independent domains that are capable of mediating transcriptional repression (RD1, RD2 and RD3).
The C-terminal region contains two separate nuclear receptor-interacting domains (ID1 and ID2), each of which contains a conserved sequence referred to as the CORNR box. This motif is necessary and sufficient for binding to unligated nuclear hormone receptors, while sequences flanking the CORNR box determine the precise nuclear hormone receptor specificity. -
Post-translational
modificationsUbiquitinated; mediated by SIAH2 and leading to its subsequent proteasomal degradation. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 9611 Human
- Entrez Gene: 20185 Mouse
- Entrez Gene: 54299 Rat
- Entrez Gene: 398304 Xenopus laevis
- Omim: 600849 Human
- SwissProt: O75376 Human
- SwissProt: Q60974 Mouse
- SwissProt: Q9WUB5 Rat
see all -
Alternative names
- hN CoR antibody
- hNCoR antibody
- KIAA1047 antibody
see all
Images
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ab3482 labelling Nuclear Receptor Corepressor NCoR in the nucleus of Mouse breast tissue (right) compared with a negative control (left) by Immunohistochemistry (formalin/PFA-fixed paraffin-embedded sections). To expose target proteins, antigen retrieval method was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Tissue was blocked in 3% H2O2-methanol for 15 min at room temperature, then incubated with primary antibody (1:200 in 3% BSA-PBS) overnight at 4°C. A HRP-conjugated anti-rabbit was used as the secondary antibody, followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
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ab3482 labelling Nuclear Receptor Corepressor NCoR in the nucleus of Human breast carcinoma (right) compared with a negative control (left) by Immunohistochemistry (formalin/PFA-fixed paraffin-embedded sections). To expose target proteins, antigen retrieval method was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Tissue was blocked in 3% H2O2-methanol for 15 min at room temperature, then incubated with primary antibody (1:200 in 3% BSA-PBS) overnight at 4°C. A HRP-conjugated anti-rabbit was used as the secondary antibody, followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
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ab3482 labelling Nuclear Receptor Corepressor NCoR (green) in the nucleus and cytoplasm of MCF-7 cells by Immunocytochemistry/Immunofluorescence. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with the primary antibody (1:200 in 3% BSA-PBS) overnight at 4 ºC. A DyLight-conjugated anti-rabbit was used as the secondary antibody. Red (phalloidin) - F-actin, Blue - nuclei. Images were taken at a magnification of 60x.
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ab3482 labelling Nuclear Receptor Corepressor NCoR (green) in the nucleus and cytoplasm of HeLa cells by Immunocytochemistry/Immunofluorescence. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with the primary antibody (1:200 in 3% BSA-PBS) overnight at 4 ºC. A DyLight-conjugated anti-rabbit was used as the secondary antibody. Red (phalloidin) - F-actin, Blue - nuclei. Images were taken at a magnification of 60x.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (15)
ab3482 has been referenced in 15 publications.
- Qin D et al. Lgr5+ cell fate regulation by coordination of metabolic nuclear receptors during liver repair. Theranostics 12:6130-6142 (2022). PubMed: 36168631
- Wang Y et al. Discovery of LAMP-2A as potential biomarkers for glioblastoma development by modulating apoptosis through N-CoR degradation. Cell Commun Signal 19:40 (2021). PubMed: 33761934
- Garg M et al. Cardiolipin-mediated PPAR? S112 phosphorylation impairs IL-10 production and inflammation resolution during bacterial pneumonia. Cell Rep 34:108736 (2021). PubMed: 33567272
- Beilner D et al. Nuclear receptor corepressor (NCoR) is a positive prognosticator for cervical cancer. Arch Gynecol Obstet 304:1307-1314 (2021). PubMed: 33861372
- St-Jean S et al. NCOR1 Sustains Colorectal Cancer Cell Growth and Protects against Cellular Senescence. Cancers (Basel) 13:N/A (2021). PubMed: 34503224