Question (13348) | Anti-Nuclear Receptor Corepressor NCoR antibody - ChIP Grade (ab3482)

Go to datasheet (ab3482)

Question

What is recommended to use a positive control for Western blotting with this antibody?

Answer

Thank you for your enquiry. Ab3482 was characterized in Western blotting using HeLa cell extract; by Western blot, this antibody detects a protein representing N-CoR from HeLa cell extracts. HeLa cell extract is therefore recommended as a positive control for this antibody. This antibody has so far been tested for cross-reactivity with human and has not yet been tested in other species. The datasheet previously said mouse, but I have updated it to reflect this new information that I obtained from the antibody's originator. If you have any additional questions, please let us know. We are still working on your question regarding ab84 and will get back to you soon.

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