Overview

  • Product name
    Nuclear yellow (Hoechst S769121)
  • Tested applications
    Suitable for: Flow Cyt, ICC/IFmore details
  • General notes
    The Hoechst stains are a family of fluorescent stains for labeling DNA used in fluorescence microscopy and flow cytometry. Because these fluorescent stains label DNA, they are also commonly used to visualize nuclei and mitochondria.

    The long-wavelength tracer Nuclear yellow is often combined with the popular retrograde tracer True Blue for two-color neuronal mapping. In neuronal cells, Nuclear yellow primarily stains the nucleus with yellow fluorescence, whereas True Blue is a UV light–excitable, divalent cationic dye that stains the cytoplasm with blue fluorescence. Both Nuclear yellow and True Blue are stable when subjected to immunohistochemical processing and can be used to photoconvert DAB into an insoluble, electron-dense reaction product.

    Molecular Weight: 651.01
    Appearance: Light yellow powder
    Solvents: water or dimethylsulfoxide (DMSO)

    SPECTRAL PROPERTIES:

    Excitation: Exmax 355 nm

    Emission: Emmax 495 nm*

Properties

Applications

Our Abpromise guarantee covers the use of ab138903 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.

Images

  • ICC/IF image of ab6046 stained HeLa cells. The cells were 100% methanol fixed and then incubated in 1% BSA / 10% normal donkey serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilize the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab6046, 1µg/ml) overnight at +4°C. The secondary antibody (orange) was ab150064 Alexa Fluor® 594 donkey anti-rabbit IgG (H+L) used at 2µg/ml for 1h. Nuclear Yellow (Hoechst S769121) (ab138903) was used to stain the cell nuclei (purple/blue) at a 250µg/ml dilution with a 60 minute incubation.
  • Chemical structure of ab138903 Nuclear yellow (Hoechst S769121).

    Chemical structure of ab138903 Nuclear yellow (Hoechst S769121).

Protocols

References

This product has been referenced in:
  • Hillje AL  et al. TRIM32-dependent transcription in adult neural progenitor cells regulates neuronal differentiation. Cell Death Dis 4:e976 (2013). Read more (PubMed: 24357807) »
See 1 Publication for this product

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