Key features and details
- Rabbit polyclonal to Nucleolar protein 14
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-Nucleolar protein 14 antibody
See all Nucleolar protein 14 primary antibodies
DescriptionRabbit polyclonal to Nucleolar protein 14
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Mouse, Human
Synthetic peptide within Human Nucleolar protein 14 aa 750-800. The exact sequence is proprietary. (NP_003694.1)
Database link: P78316
- WB: HeLa and NIH/3T3 whole cell lysate.
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesAntibody was affinity purified using an epitope specific to Nucleolar protein 14 immobilized on solid support.
Our Abpromise guarantee covers the use of ab241495 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000.|
FunctionInvolved in nucleolar processing of pre-18S ribosomal RNA. Has a role in the nuclear export of 40S pre-ribosomal subunit to the cytoplasm.
Sequence similaritiesBelongs to the NOP14 family.
Cellular localizationNucleus > nucleolus.
- Information by UniProt
- C4orf9 antibody
- chromosome 4 open reading frame 9 antibody
- NOL14 antibody
All lanes : Anti-Nucleolar protein 14 antibody (ab241495) at 0.1 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate at 50 µg
Lane 2 : HeLa cell lysate at 15 µg
Developed using the ECL technique.
Exposure time: 3 minutes
All lanes : Anti-Nucleolar protein 14 antibody (ab241495) at 0.4 µg/ml
Lane 1 : NIH/3T3 (mouse embryo fibroblast cell line) cell lysate at 50 µg
Lane 2 : NIH/3T3 cell lysate at 15 µg
Developed using the ECL technique.
Exposure time: 10 seconds
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab241495 has not yet been referenced specifically in any publications.