Anti-Nucleolar protein 14 antibody (ab72741)
Key features and details
- Mouse polyclonal to Nucleolar protein 14
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Nucleolar protein 14 antibody
See all Nucleolar protein 14 primary antibodies -
Description
Mouse polyclonal to Nucleolar protein 14 -
Host species
Mouse -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein within Human Nucleolar protein 14. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
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Positive control
- Human pancreas tissue lysate and Nucleolar protein 14 transfected 293T cell lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.40
Constituent: 100% PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab72741 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/1000. Detects a band of approximately 89, 105 kDa (predicted molecular weight: 91 kDa).
Predicted molecular weight for isoform 2: 91 kDa. |
Notes |
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WB
1/500 - 1/1000. Detects a band of approximately 89, 105 kDa (predicted molecular weight: 91 kDa). Predicted molecular weight for isoform 2: 91 kDa. |
Target
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Function
Involved in nucleolar processing of pre-18S ribosomal RNA. Has a role in the nuclear export of 40S pre-ribosomal subunit to the cytoplasm. -
Sequence similarities
Belongs to the NOP14 family. -
Cellular localization
Nucleus > nucleolus. - Information by UniProt
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Database links
- Entrez Gene: 8602 Human
- GenBank: BC026035 Human
- GenBank: NP_003694.1 Human
- Omim: 611526 Human
- SwissProt: P78316 Human
- Unigene: 627133 Human
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Alternative names
- C4orf9 antibody
- chromosome 4 open reading frame 9 antibody
- NOL14 antibody
see all
Images
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Anti-Nucleolar protein 14 antibody (ab72741) at 1/500 dilution + human pancreas tissue lysate at 25 µg
Secondary
Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution
Predicted band size: 91 kDa
Observed band size: 89 kDa why is the actual band size different from the predicted? -
All lanes : Anti-Nucleolar protein 14 antibody (ab72741) at 1/500 dilution
Lane 1 : Nucleolar protein 14 transfected 293T cell lysate
Lane 2 : Non-transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution
Predicted band size: 91 kDa
Observed band size: 105 kDa why is the actual band size different from the predicted?
Datasheets and documents
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Datasheet download
References (0)
ab72741 has not yet been referenced specifically in any publications.