Key features and details
- Assay type: Cell-based
- Platform: Fluorescence microscope
- Assay time: 30 min
- Sample type: Adherent cells, Suspension cells
Product nameNucleolar Staining Kit - Green Fluorescence - Cytopainter
Sample typeAdherent cells, Suspension cells
Assay time0h 30m
Species reactivityReacts with: Mammals, Other species
Nucleolar Staining Kit - Green Fluorescence | Cytopainter (ab139475) contains a proprietary dye suitable for live-cell staining of nucleoli. The dye allows examination of nucleolar dynamic changes in intracellular distribution, trafficking and localization arising from biological processes such as the cell cycle and ribosome biogenesis. The kit is compatible with most fluorescence detection systems, including conventional and confocal fluorescence microscopes, as well as High Content Screening (HCS) platforms.
ab139475 is specifically designed for visualizing nucleoli in living cells. The dye in the kit is resistant to photobleaching, facilitating its use in imaging applications. This nucleolar dye may be used in conjunction with common live-cell nuclear counterstains such as Hoechst 33342, Draq5™ or Nuclear Red dye.
A control nucleolus perturbation agent, actinomycin D, is provided for monitoring changes in nucleolar dynamics. Potential applications for this kit include monitoring impaired ribosome biogenesis, inhibition of transcription, cell cycle dynamics and cellular stress, as well as the distribution, trafficking and dynamics of nucleolar proteins, the distribution of viral proteins, and potentially as an aid in identifying cancer cells.
ab139475 has been referenced in 1 publication.
- Maass PG et al. Spatiotemporal allele organization by allele-specific CRISPR live-cell imaging (SNP-CLING). Nat Struct Mol Biol 25:176-184 (2018). PubMed: 29343869