Overview

  • Product name

    Nucleolar Staining Kit - Green Fluorescence - Cytopainter
  • Sample type

    Adherent cells, Suspension cells
  • Assay type

    Cell-based
  • Assay time

    0h 30m
  • Species reactivity

    Reacts with: Mammals, Other species
  • Product overview

    Nucleolar Staining Kit - Green Fluorescence | Cytopainter (ab139475) contains a proprietary dye suitable for live-cell staining of nucleoli. The dye allows examination of nucleolar dynamic changes in intracellular distribution, trafficking and localization arising from biological processes such as the cell cycle and ribosome biogenesis. The kit is compatible with most fluorescence detection systems, including conventional and confocal fluorescence microscopes, as well as High Content Screening (HCS) platforms.


    ab139475 is specifically designed for visualizing nucleoli in living cells. The dye in the kit is resistant to photobleaching, facilitating its use in imaging applications. This nucleolar dye may be used in conjunction with common live-cell nuclear counterstains such as Hoechst 33342, Draq5™ or Nuclear Red dye.


    A control nucleolus perturbation agent, actinomycin D, is provided for monitoring changes in nucleolar dynamics. Potential applications for this kit include monitoring impaired ribosome biogenesis, inhibition of transcription, cell cycle dynamics and cellular stress, as well as the distribution, trafficking and dynamics of nucleolar proteins, the distribution of viral proteins, and potentially as an aid in identifying cancer cells.


    Review other dyes and kits for nucleolar staining, or the live cell staining fluorescent dyes guide

  • Platform

    Fluorescence microscope

Properties

Images

  • Absorption and fluorescence emission spectra for Green Detection Reagent dye. Spectrum was determined in 1X Assay Buffer.

Protocols

References

This product has been referenced in:

  • Maass PG  et al. Spatiotemporal allele organization by allele-specific CRISPR live-cell imaging (SNP-CLING). Nat Struct Mol Biol 25:176-184 (2018). Read more (PubMed: 29343869) »
See 1 Publication for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Abreviews
The cytopainter Nucleolar Staying Kit-Green Fluorescence has been tested with a standard PFA kai TritonX-100 immunofluorescence protocol, for ki67 a nucleolar staining antibody. The green nucleolar dye cannot be used for IF, given that the signal of the nucleolar staining was low in comparison with the high background, throughout the cell.
The product has been tested as a part of an AbTrial scheme, with a discount code :

Dr. Dimitra Kalamida

Verified customer

Submitted Aug 27 2014

Answer

CytoPainter Nucleolar Staining Kit - Green Fluorescence, ab139475 has not been validated for antibody staining following staining of nucleolus. Since we have not tested for antibody staining we do not know what to expect. We are hoping that the protocols would work although we are not sure permeabilization may cause loss of fluorescence intensity.



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