Key features and details
- Assay type: Cell-based
- Platform: Fluorescence microscope
- Assay time: 30 min
- Sample type: Adherent cells, Suspension cells
Product nameNucleolar/Nuclear Staining Kit - Green/ Red Fluorescence - Cytopainter
Sample typeAdherent cells, Suspension cells
Assay time0h 30m
Species reactivityReacts with: Mammals, Other species
Nucleolar/Nuclear Staining Kit - Green/ Red Fluorescence | Cytopainter (ab139474) contains two proprietary dyes suitable for simultaneous live-cell staining of nucleoli and nuclei. The dyes allow examination of nucleolar dynamic changes in intracellular distribution, trafficking and localization arising from biological processes such as the cell cycle and ribosome biogenesis. The kit is compatible with most fluorescence detection systems, including conventional and confocal fluorescence microscopes, as well as High Content Screening (HCS) platforms.
This kit is specifically designed for visualizing nucleoli and nuclei in living cells. A control nucleolus perturbation agent, actinomycin D, is provided for monitoring changes in nucleolar dynamics. Potential applications for this kit include monitoring impaired ribosome biogenesis, inhibition transcription, cell cycle dynamics and cellular stress, as well as the distribution, trafficking and dynamics of nucleolar proteins, the distribution of viral proteins, and potentially as an aid in identifying cancer cells.
ab139474 has been referenced in 1 publication.
- Ramamurthy B et al. Three-Dimensional Cellular Raman Analysis: Evidence of Highly Ordered Lipids Within Cell Nuclei. J Histochem Cytochem 66:889-902 (2018). PubMed: 30138043