Overview

  • Product name

    Anti-Nucleolin antibody [364-5] (Alexa Fluor® 488)
    See all Nucleolin primary antibodies
  • Description

    Mouse monoclonal [364-5] to Nucleolin (Alexa Fluor® 488)
  • Host species

    Mouse
  • Conjugation

    Alexa Fluor® 488. Ex: 495nm, Em: 519nm
  • Tested applications

    Suitable for: ICC/IF, Flow Cytmore details
  • Species reactivity

    Reacts with: Human
    Does not react with: Mouse, Rat
  • Immunogen

    Tissue, cells or virus corresponding to Human Nucleolin.

  • Positive control

    • HDFn and HeLa cells.
  • General notes

    Product was previously marketed under the MitoSciences sub-brand.

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Properties

Applications

Our Abpromise guarantee covers the use of ab154028 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 0.2 µg/ml.
Flow Cyt Use a concentration of 1 µg/ml.

ab171463 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

Target

  • Function

    Nucleolin is the major nucleolar protein of growing eukaryotic cells. It is found associated with intranucleolar chromatin and pre-ribosomal particles. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly. May play a role in the process of transcriptional elongation. Binds RNA oligonucleotides with 5'-UUAGGG-3' repeats more tightly than the telomeric single-stranded DNA 5'-TTAGGG-3' repeats.
  • Sequence similarities

    Contains 4 RRM (RNA recognition motif) domains.
  • Post-translational
    modifications

    Some glutamate residues are glycylated by TTLL8. This modification occurs exclusively on glutamate residues and results in a glycine chain on the gamma-carboxyl group.
  • Cellular localization

    Nucleus > nucleolus. Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs.
  • Information by UniProt
  • Database links

  • Alternative names

    • C23 antibody
    • FLJ45706 antibody
    • MS1116 antibody
    • NCL antibody
    • Nucl antibody
    • NUCL_HUMAN antibody
    • Nucleolin antibody
    • Protein C23 antibody
    see all

Images

  • Immunocytochemistry with HeLa cells were stained with Anti-Nucleolin-488 antibody (0.2 µg/mL, ab154028) in green, Anti-GAPDH (1.0 µg/mL, ab110305) as red, and DAPI (1/10,000) in blue, as a nuclear stain. Secondary antibody used was goat anti-mouse IgG1 Alexa-594 at 1/1000 dilution. A) HeLa cells 40x. B) HeLa cells 100x.
  • Immunocytochemistry with HDFn cells were stained with Anti-Nucleolin-488 antibody (0.2 µg/mL, ab154028) in green, Anti-GAPDH (1.0 µg/mL, ab110305) as red, and DAPI in blue, as a nuclear stain. Secondary antibody used was goat anti-mouse IgG1 Alexa-594 at 1/1000 dilution. A) HDFn 40x. B) HDFn 100x.
  • Flow Cytometry for Anti-Nucleolin-488 antibody (ab154028) stained HeLa cells. Flow cytometry results of unstained cells (black) and 1.0 µg/mL Nucleolin-488 (red) stained cells.

References

This product has been referenced in:

  • Roy S  et al. Optimization of prostate cancer cell detection using multiplex tyramide signal amplification. J Cell Biochem 120:4804-4812 (2019). Read more (PubMed: 30390333) »
  • Martins RP  et al. Nuclear processing of nascent transcripts determines synthesis of full-length proteins and antigenic peptides. Nucleic Acids Res N/A:N/A (2019). Read more (PubMed: 30624716) »
See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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