Overview

  • Product name

    Anti-Nucleolin antibody [364-5] - BSA and Azide free
    See all Nucleolin primary antibodies
  • Description

    Mouse monoclonal [364-5] to Nucleolin - BSA and Azide free
  • Host species

    Mouse
  • Tested applications

    Suitable for: IHC-P, Flow Cyt, WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Does not react with: Mouse, Rat, Cow
  • Immunogen

    Tissue, cells or virus corresponding to Human Nucleolin. (Lysate of SU-DHL-1 nuclei).

  • Positive control

    • Human skin and tonsil tissues; Testis, ovary, uterus, endometrial and Hodgkin’s lymphoma tissues.

Properties

Applications

Our Abpromise guarantee covers the use of ab212672 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 0.25 - 0.5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

(Primary incubation for 30 minutes at RT).

Flow Cyt Use 0.5-1µg for 106 cells.

(in 0.1ml).

WB Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 76 kDa.
ICC/IF Use a concentration of 0.5 - 1 µg/ml.

Target

  • Function

    Nucleolin is the major nucleolar protein of growing eukaryotic cells. It is found associated with intranucleolar chromatin and pre-ribosomal particles. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly. May play a role in the process of transcriptional elongation. Binds RNA oligonucleotides with 5'-UUAGGG-3' repeats more tightly than the telomeric single-stranded DNA 5'-TTAGGG-3' repeats.
  • Sequence similarities

    Contains 4 RRM (RNA recognition motif) domains.
  • Post-translational
    modifications

    Some glutamate residues are glycylated by TTLL8. This modification occurs exclusively on glutamate residues and results in a glycine chain on the gamma-carboxyl group.
  • Cellular localization

    Nucleus > nucleolus. Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs.
  • Information by UniProt
  • Database links

  • Alternative names

    • C23 antibody
    • FLJ45706 antibody
    • MS1116 antibody
    • NCL antibody
    • Nucl antibody
    • NUCL_HUMAN antibody
    • Nucleolin antibody
    • Protein C23 antibody
    see all

Images

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded human skin tissue labeling Nucleolin with ab212672 at 0.5 µg/ml.

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded human tonsil tissue labeling Nucleolin with ab212672 at 0.5 µg/ml.

References

ab212672 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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