Anti-Nucleolin antibody (ab50279)
Key features and details
- Rabbit polyclonal to Nucleolin
- Suitable for: WB, Flow Cyt, ICC/IF, IP
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-Nucleolin antibody
See all Nucleolin primary antibodies -
Description
Rabbit polyclonal to Nucleolin -
Host species
Rabbit -
Tested applications
Suitable for: WB, Flow Cyt, ICC/IF, IPmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Cynomolgus monkey, Orangutan -
Immunogen
Synthetic peptide:
VKLAKAGKNQGDPKKMA
conjugated to KLH, corresponding to N terminal amino acids 2-17 of Human Nucleolin with a C-terminal added cysteine. -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: 0.0268% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
This antibody has been affinity-purified using the immunizing peptide immobilized on agarose. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab50279 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use at an assay dependent concentration.
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Flow Cyt |
Use at an assay dependent concentration. PubMed: 20814900
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
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ICC/IF |
Use a concentration of 10 - 20 µg/ml.
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IP |
Use at an assay dependent concentration.
1-2 ug/ml immunoprecipitates Nucleolin from 250 ug RIPA lysate of human HeLa cells. |
Notes |
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WB
Use at an assay dependent concentration. |
Flow Cyt
Use at an assay dependent concentration. PubMed: 20814900 ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
ICC/IF
Use a concentration of 10 - 20 µg/ml. |
IP
Use at an assay dependent concentration. 1-2 ug/ml immunoprecipitates Nucleolin from 250 ug RIPA lysate of human HeLa cells. |
Target
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Function
Nucleolin is the major nucleolar protein of growing eukaryotic cells. It is found associated with intranucleolar chromatin and pre-ribosomal particles. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly. May play a role in the process of transcriptional elongation. Binds RNA oligonucleotides with 5'-UUAGGG-3' repeats more tightly than the telomeric single-stranded DNA 5'-TTAGGG-3' repeats. -
Sequence similarities
Contains 4 RRM (RNA recognition motif) domains. -
Post-translational
modificationsSome glutamate residues are glycylated by TTLL8. This modification occurs exclusively on glutamate residues and results in a glycine chain on the gamma-carboxyl group. -
Cellular localization
Nucleus > nucleolus. Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. - Information by UniProt
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Database links
- Entrez Gene: 101865794 Cynomolgus monkey
- Entrez Gene: 4691 Human
- Entrez Gene: 17975 Mouse
- Entrez Gene: 100174230 Orangutan
- Entrez Gene: 25135 Rat
- Omim: 164035 Human
- SwissProt: Q4R4J7 Cynomolgus monkey
- SwissProt: P19338 Human
see all -
Alternative names
- C23 antibody
- FLJ45706 antibody
- MS1116 antibody
see all
Images
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (25)
ab50279 has been referenced in 25 publications.
- Lappin KM et al. Cancer-Associated SF3B1 Mutations Confer a BRCA-Like Cellular Phenotype and Synthetic Lethality to PARP Inhibitors. Cancer Res 82:819-830 (2022). PubMed: 35027467
- Kosar M et al. The human nucleoporin Tpr protects cells from RNA-mediated replication stress. Nat Commun 12:3937 (2021). PubMed: 34168151
- Pipier A et al. Constrained G4 structures unveil topology specificity of known and new G4 binding proteins. Sci Rep 11:13469 (2021). PubMed: 34188089
- Sessa G et al. BRCA2 promotes DNA-RNA hybrid resolution by DDX5 helicase at DNA breaks to facilitate their repair‡. EMBO J 40:e106018 (2021). PubMed: 33634895
- Dagg RA et al. A transcription-based mechanism for oncogenic β-catenin-induced lethality in BRCA1/2-deficient cells. Nat Commun 12:4919 (2021). PubMed: 34389725