Question (44819) | Anti-Nucleolin antibody (ab70493)

Go to datasheet (ab70493)

Question

Dear abcam technical support:

I just called minutes ago about the ab22758 antibody and I’ve received great supports from your operator. The reason I called in for technical support is because we would like to run the same test on new batch of experimental samples but we couldn’t get the apical staining back anymore as they’ve published on the paper. This problem started after we got the new lot of this antibody and it has been missing the apical staining on the mouse lungs. Even performing the same staining protocol on the samples that worked before, which were used for the paper, with this new lot antibody, the apical staining is not showing.

We had called in to asked for replacement vial but it didn’t solve the problem. As you might recalled from our conversation record, we’ve also tried ab70493 and that didn’t help as well. We’ve also been asking if there’s any new lot of the ab22758 that might be coming soon but seems like you still have a lot of vials in stock of this lot.

From our conversation today over the phone, seems like the only thing we can find out is if there’s anything unusual about the QC on this lot. Would there be anything else that might led to the variation of this lot compare to the previous? I hope you can help us find out if there’s any way that we can get the apical staining back since this is the only antibody that we had consistent success with the old lots. We’ve tried all other antibodies that target Nucleolin but nothing give us the staining we wanted.

Thank you in advance for your help

Answer

Thank you for your call today and for your email.
I apologize again for the batch variability of ab22758, and I have a couple of additional questions. Do you know which lot number was used previously to produce the apical staining? If not, do you have an order or PO number (I can look up the lot number)? This will be helpful for comparing QC data.
Also, do you have any images of the most recent staining with this lot?
I look forward to hearing from you and I'm hopeful that we can resolve this issue. Please let me know if you have any questions or if there is anything else that we can do for you.