Question (49455) | Anti-Nucleolin antibody (ab70493)

Sorry that it took me this long to reply your email, I was occupied with my work and I need some time to gather some information that you requested about my inquiry. I had taken two pictures on the IHC staining that I did on mouse lungs with ab22758 nucleolin antibody in separate time. The first one was done in January 2010, lot number was 724414, and this slide shows the staining that we expected on the apical aspect of the bronchioles. I have added some red arrows to point out the apical staining, which looks like someone outlines the lining using a marker. The second slide was done early this year, lot number GR8557-2 and the staining was done using the same protocol as the previous, and all the apical staining were not detected. This apical staining should appear in the mouse lung bronchioles. I have added some red arrows on this second slide too to indicate where the apical staining supposed to be. They were not picked up by the antibody but instead stained in blue by the hematoxylin.
I hope this will be sufficient information for you to investigate what might be the problem between these two lots and please contact me anytime if you need additional information. Thank you again for your time in this matter.