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Sorry that it took me this long to reply your email, I was occupied with my work and I need some time to gather some information that you requested about my inquiry. I had taken two pictures on the IHC staining that I did on mouse lungs with ab22758 nucleolin antibody in separate time. The first one was done in January 2010, lot number was 724414, and this slide shows the staining that we expected on the apical aspect of the bronchioles. I have added some red arrows to point out the apical staining, which looks like someone outlines the lining using a marker. The second slide was done early this year, lot number GR8557-2 and the staining was done using the same protocol as the previous, and all the apical staining were not detected. This apical staining should appear in the mouse lung bronchioles. I have added some red arrows on this second slide too to indicate where the apical staining supposed to be. They were not picked up by the antibody but instead stained in blue by the hematoxylin.
I hope this will be sufficient information for you to investigate what might be the problem between these two lots and please contact me anytime if you need additional information. Thank you again for your time in this matter.
Asked on Jun 01 2012
Thank you for your reply.
I forwarded the images and information to my colleagues in the lab, and we have only tested this antibody in tonsil tissue. My colleague who specifically works with this antibody is out of the office until next office, but we'll be able to take a look at the previous data once she returns.
The lab is also offering to test a couple of the current lots in mouse lung to see if one works better; would you like us to do this? I'm not sure what the time frame would be to get the results, but I would send them to you once they're available.
Please let me know if you would be interested in this and I will set it up with the lab. I look forward to hearing from you. Have a great weekend!
Answered on Jun 01 2012